高产单宁酶的黑曲霉菌株鉴定及其固态发酵产酶条件优化

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中图法分类号：TQ925.2 文献标识码：A 文章编号：1000-2324（2025）04-0670-11

Identification of High Tannase-producing Aspergillus niger Strains and Optimization of Solid-state Fermentation Conditions for Enzyme Production

WANG Xin-yu'， LI Hai-peng'， SONG Da-peng²， SUN Xia²， WANG Kun-peng². FANG Feng-xiang²， DING Shi-bo²*， YANG Feng-shan1*

1.EngineeringResearch CenterofAgriculturalMicrobiologyTechnology/MinistryofEducation，HeilongjiangProvincial KeyLaboratoryofEcologicalRestorationandResourceUtilizationforColdRegion，KeyLaboratoryofMolecularBiology/ CollegeofHeilongjiangProvince，choolofifeciences/HeilongjiangUniversity，Harbin5oo8o，Cina 2.RizhaoAcademy ofAgricultural Sciences，Rizhao27680o，China

Abstract：This studyisolatesand screensa fungal strainfromtealeavesusingatannase identification medium.The strain is identifiedasAspergillusnigerthroughmorphologicalcharacteristicsandITSsequenceanalysis，anddesignatedasstrain ALF1-1.FermentedwiththesporesuspensionofstrainALF1-1，thetanaseproductionactivityofblack tealeafstalks reaches 10.42U/mL .Subsequently，the optimal fermentation conditions are determined through one-factor and response surface experiments，which are as follows： temperature 31∘C ，incubation time ，inoculum size 4% ，andammonium chloride concentration 7% .Under these conditions，the predicted enzyme activity is 24.9U/mL ，while the measured activity reaches 25.92U/mL .Compared to the pre-optimization enzyme activity （10.4U/mL ），the optimized solid-state fermentation conditions enhance tannase production by strain ALF11-1 approximately 2.49-fold.

Keywords： Tea plant; tannase;Aspergillus niger; response surface

单宁酶（Tannase，EC.3.1.1.20），全称为单宁酰基水解酶，是一种能够水解单宁中酯键和缩酚羧键的酶，其基本功能是将单宁水解成没食子酸和葡萄糖[。（剩余12347字）