发光金属有机框架和碳点嵌入分子印迹比率荧光探针对恩诺沙星检测

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中图法分类号：TS201.6 文献标识码：A 文章编号：1000-2324（2025）04-0566-07

Luminescent Metal-organic Frameworks and Carbon Dotsembedded Molecular Imprinted Ratiometric Fluorescent Probe forEnrofloxacin Detection

CHAI Ying-xin1， QI Qing-ying1，LI Xiao-fei²， XULong-hual

1.CollegeofFoodScienceandEngineering/ShandongAgriculturalUniversityai'an278，China

2.Tai'anCityCentralHospital，Tai'an27oo，Cina

Abstract：Long-termintakeoffoods with excessveresiduesofenrofloxacin （ENR）posesathreat tohumanhealth，and itis importanttoestablisharapidandhighlysensitivedetectiontechnologytostrengthen themonitoringofitsresiduelevel.This paper synthesized europium-based luminescent metal-organic frameworks （Eu-MOFs）and carbondots （CDs），and prepared molecularly imprinted sol-gel technology，Eu-MOFand CDs-embedded molecularly imprintedpolymer （europium-based luminescent metal-organic frameworks and carbon dots-embedded molecular imprinted polymer， Eu-MOF/CDs@MIP） ratiometric fluorescent probebycombiningmolecularimprintingsol-gel technology.Thematerialswerecharacterized by scaning electronmicroscopy，transmissionelectronmicroscopy，andFourier transform infrared spectroscopy.andtheEuMOF/CDs@MIP probe were applied to the detection of real samples.Theresults show that Eu-MOF/CDs@MIP is a homogeneous spherical structure with stable fluorescence emision，fast mass transferrate and high saturationcapacity. Enrofloxacin significantly enhanced the fluorescence emission of Eu-MOF at 615nm ，while the fluorescence of carbon dots at 430nm remained largely unchanged.Basedon the linear relationship between the ratio of the two fluorescence intensities andtheconcentrationofenrofloxacin，anewmethodforthehighlyselectiveandsensitivedetectionofenrofloxacinwas established. The linear range of the method was 0.03～20.00mg-1 ，the lowest detection limit was 0.01mg⋅L-1 ，andthe recoverieswere 85.0%～108.8% in real samples.

Keywords：Ratiometric fluorescentprobe；luminescent metal-organic frameworks；carbondots；molecular imprinting; enrofloxacin

恩诺沙星为第三代氟喹诺酮类抗生素，具有价格低廉、副作用发生率低、抗菌谱广、杀菌活性强等特点[1]，曾被广泛应用于畜牧业和水产养殖业。（剩余10403字）