白三叶抗旱种质资源筛选及抗旱评价指标筛选

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中图分类号：S541.2 文献标识码：A 文章编号：1007-0435（2025）05-1510-13

Abstract：To identify white clover （Trifolium repens） germplasm resources with exceptional drought resistance and to select efective indicators for evaluating this trait，the present study was conducted using 11O T.repens germplasm resources from natural distributions in Sichuan and other regions，along with the nationally recognized cultivars‘Haifa’and‘Goethe’as test materials.Under natural drought conditions in the field，a prelimi nary evaluation of drought resistance Was performed by observing the differences in phenotypic indices of the test materials.In controlled indoor conditions，the weighted subordinate function method was employed to assess the drought resistance of the initially screened materials by examining changes in the cross-sectional structure of the leaf blades and various physiological parameters.Correlation and principal component analyses were utilized to evaluate thecontribution rate of each index，which facilitated the identification of suitable indicators for assessing the drought resistance of white clover.The results indicated a close relationship between the mor phological characteristics of white clover materials and their drought tolerance，as revealed by cluster analysis. The comprehensive evaluation identified materials Tr210 ，TrlO3，and TrO83 as having strong drought toler ance，while TrO08 and Tr033 exhibited poor drought tolerance. Among the 26 evaluation indices，16 effective indices were ultimately selected，comprising 4 phenotypic indices，8 physiological indices，and 4 leaf micro structure indices.This study provides a theoretical foundation and material basis for the evaluation of droughttolerant germplasm resources，as wellas for the selection and breeding of drought-resistant varieties of white clover.

Key words： Trifolicem repens;Drought resistance;Drought resistance index;Comprehensive evaluation

干旱会导致植物的正常生长发育受到严重限制，已成为当前全球农业生产面临的最大非生物胁迫之一[1]。（剩余16870字）