石斛属灯笼组物种叶绿体基因组特征及系统发育分析

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中图分类号：Q943.2 文献标识码：A 文章编号：1007-0435（2025）05-1372-15

Abstract：To analyze the chloroplast（CP）genome structure and phylogenetic relationships of section Densiflora i the genus of Dendrobium，this studyused high-throughput sequencing technology for the first time to sequence the CP genomes of four species in the section，including D . farmer，D. palpebrae，D. sulcatum，and D.thyrsiflorum. The genomes of section Densiflora were assembled，annotated，mapped and analyzed by bioinformatics software.Phylogenetic trees of 42 species of Dendrobium，including ten species of section Densiflora，were constructed based on cp genomes. The complete cp genomes of section Densiflora were 151 717- 160 123 bp in size with 37.1%-37.6% GC content. Additionally，46-63 simple sequence repeats（SSRs） and 48-49 interspersed nuclear elements （INEs）were identified in section Densiflora. Codon usage in proteincoding genes is biased toward A and U（or T）.There were significant differences inthe coding regions of six genes， including trnKUUU ， atϕF ，rpoC， trnLUAA ，clpP and among section Densiflora. Phylogenetic analyses revealed that ten species of section Densiflora were clustered into four diferent clades，indicating that this section defined by traditional morphology is non-monophyletic. Key words：Dendrobium section Densiflora;Chloroplast genome;Genomic comparison;Phylogenetic analysis

石斛属（Dendrobium）隶属兰科（Orchidaceae）石斛亚族（Dendrobiinae），与石豆兰属（Bulbophyl-lum）构成姐妹类群[1]。（剩余20130字）