羊栖菜多酚抗氧化及细胞损伤保护作用研究

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中图分类号：TS201.1 文献标志码：A 文章编号：1001-5779（2025）08-0733-06

DOI：10.3969/j.issn. 1001-5779.2025.08.002

Abstract：Objective：Exploring theinvitroantioxidantactivityof polyphenols from Sargassum fusiformeand their protective effects against H22 -induced damage in human aortic endothelial cells（HAECs），thereby providinga theoretical basisfortheirapplicationanddevelopmentinfunctional foodandpharmaceutical fields.Methods ： Polyphenols from Sargassum fusiforme were extracted from Sargassum fusiforme with 60 % ethanol，followed by defatted with petroleumetherandpurificationviasilicagelcolumnchromatography.Theinvitroantioxidantcapacitywasassessed by measuring scavenging activitiesagainst1，1-diphenyl-2-picrylhydrazyl（DPPH） radicals，superoxide anion（ J2- ） radicals，and 2，2′ -azino-bis（3-ethylbenzothiazoline-6-sulfonic acid）（ABTS+）radicals.An oxidative damage model was established in HAECs induced by H202 toevaluate the effects ofpolyphenols from Sargassum fusiforme oncell viability andanalyze the dose-response relationship.Results：Thepolyphenols from Sargassum fusiforme showed significant scavenging ability to all three free radicals，and its half-maximal inhibitory concentration（ IC50 ）was：DPPH radical （204号 0.074mg*mL-1 ， O2- radical 0.069mg∙mL-1 ，ABTS+radical 0. 089mg⋅mL-1 .Incellular experiments，polyphenols from Sargassum fusiformealleviated H2O2 -induced oxidative damage in HAECsin a concentration-dependent mannerand significantlyenhancedcell viability（ P<0.05 ）.Conclusion：Thepolyphenols fromSargassum fusiforme exhibit potent in vitroantioxidantactivityandobviouscytoprotection.Theyactasnaturalantioxidants forfunctional foodsorbiomedical applications to prevent oxidative stress-related diseases.

Key Words：Sargassum fusiforme;Polyphenols；Antioxidant activity；Cell damage

氧化应激是细胞损伤和多种疾病发生发展的核心病理机制之一。（剩余10242字）