青海地区藏茴香种质资源遗传多样性的ISSR分析

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ISSR Analysis of Genetic Diversity of Carum carvi L. Germplasm Resources in Qinghai Region

ZHENGMing-xian，YANGLi-na，HENNing-dongetal（ColegeofAgrcultureandAnimalHusbandryQinghai Universityining， Qinghai 810016）

AbstractObjective]Toexplorethegeneticdiversityandgeneticelationshipof15Carumcari germplasmsinQinghairegionandtopro vide theoretical support for the artificial cultivation of carvivarieties.[Method] DNA from 149 fresh leaves of C . carvi was extracted by modifiedCTABmethod，andthn10igeffciencISprimerswereusedfomleularlabeling.Result]Atotalof8412plymoicbnd were obtained，the total polymorphism ratio was 90.21% ，the average Nei’s gene diversity index （ ）was O.5O，the average Shannon’s information index （I） was O.75，and the genetic similarity coefficient among C . carvi germplasm was 0.84-0. 97. The genetic diversity index ）， intra-population genetic diversity index（ ），gene differentiation coefficient （ ）and gene flow （ ）were0.23，0.17，0.26 and1.42，respectively.Te5gerplasswerdvidedintothecategois.e49C.carindivualseredividdinto6ategories.Cocusione germplasm of wild .carvi in Qinghaiareahasrich geneticdiversityandthere isextensivegeneexchange between germplasms，ndthis gene exchangeshowsacertain gradual difusion lawinthesame direction intheregion，andtherichgenetic diversityof wild C . carvi in Qinghai area comes from the differences between individuals.

Key WordsCarum carvi L. ；Germplasm resource；ISSR molecular marker；Genetic diversity

藏茴香（CarumcarviL.），常生长于路旁、草原、山沟、河滩、山坡等处的二年或多年生草本植物，属伞形科（Umbellif-erae）葛缕子属（Carum），又名葛缕子，在青藏高原俗称郭鸟、马缨子[1]，种子呈长卵圆形，表面有纵棱，种皮深褐色[2]。（剩余8250字）