多花黄精叶斑病病原鉴定及拮抗菌对其抑制作用研究

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IdentificationofPathogensofPolygonatummultiflorumLeaf SpotDiseaseandTheirInhibitionbyAntagonistic Bacteria HUXiu-hong1，HEJaZHANGTing-ietal（1.SholofLifeandHealthSience，KailiUniversityKailiGuzou556；.Qiandongnan Food and Drug Inspection and Testing center，Kaili，Guizhou ）

AbstractUsigplantswithtypicalsymptomsofPlygonatummultfomlafspotdiseaseasrawmaterials，tepatogenicfungiwereisolatedfromthediseasedleavesusingtisueisolationmetodMorphologicalobservationandequenceanalysisofribosoalDA-（nteal transcribedspacer）regionwereusedtoasifyandidentifeathogenicfungi.eibitoryectsoftheantagonisticacteiaGlu conobactereeliH-cillssubilisdcilsgm-ntpagefuieedulsot themainpathogenscausingteleafspotdiseaseofPlygoatumultiformreEicocmsorhumandPestalotiopsismicospaute searchhafondattetagosticacteuofBcilussubtilisH-3dsrogiibitoetoothransofpatogsii tion rates of 91. 37% and 98.21% ，respectively；Bacillus magnetrium B-2 also had a strong inhibitory effect with inhibition rates of 44.98% and 95.49% ，respectively；whileGluconobacternepheli H-1onthetwopathogenicfungi mentionedabove wasrelativelyweak，withinibition rates of 31.89% and 50.41% ，respectively.In summary，Bacillus subtilis has the best antibacterial effect and can provide a basis for the research and development of biopesticides in the later stage.

KeywordsPlygoatummuliforu；eafspotdisease；PathognicfungiSeparationandidentification；Antagonisticbacterabioy effect

多花黄精（PolygonatumcyrtonemaHua.）又名姜形黄精、野生姜、黄精姜等，为百合科（Liliaceae）黄精属（Polygona-tum）多年生宿根草本植物，属于传统的中药材，是药食同源植物[1-2]。（剩余6387字）