珠芽魔芋叶片组培褐变抑制条件探索

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摘要  以珠芽魔芋叶片作为外植体，通过培养温度、活性炭以及培养光暗条件的改变等简单易操作的方式，探索抑制组织褐变的最佳环境条件。结果表明，与光照相比，黑暗培养条件可以有效抑制魔芋叶片组织褐变，褐变率仅为 5.88%；温度和活性炭处理对珠芽魔芋叶片褐变的抑制效果均不佳。

关键词  珠芽魔芋；叶片；褐变

中图分类号  S 632.3   文献标识码  A   文章编号  0517-6611（2023）05-0037-04

doi： 10.3969/j.issn.0517-6611.2023.05.010

开放科学（资源服务）标识码（OSID）：

Study on Browning Inhibition of Amorphophallus bulbifer Leaf Tissue Culture

ZHANG Li-na，WANG Rui-jia，XIE Yu-qi  et al

（College of Agriculture， Yunnan University， Kunming， Yunnan 650504）

Abstract  Amorphophallus bulbifer leaves were used as explants to explore the optimal environmental conditions for inhibiting tissue browning through simple and easy operation methods such as culture temperature， activated carbon and the change of light and dark conditions. The results showed that compared with light culture， dark culture conditions could effectively inhibit the browning of konjac leaves， and the browning rate was only 5.88%.The inhibitory effects of temperature and activated carbon treatment on the browning of konjac leaves were poor.

Key words  Amorphophallus bulbifer;Leaves;Browning

珠芽魔芋是近年来已经产业化应用的一类可食用魔芋，因葡甘聚糖含量高、抗病能力强、种植范围广等优良特性，在食品、医药、工业等领域广泛应用，有较高经济价值，可作为林下经济作物种植，发展立体农业，带动山区经济增长，具有良好的产业开发与市场前景［1-3］。（剩余7548字）