C5-12启动子降低rAAV1介导的基因递送后转基因的免疫反应

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中图分类号：Q78 文献标志码：A 文章编号：1000-5013（2025）04-0435-07

C5-12 Promoter Reduces Transgene Immune Response Following rAAV1-Mediated Gene Delivery

WANG Xiao1，HUANG Xiaoping²，LI Ling1 ，ZHANG Hong1， DIAO Yong1

（1.School of Medicine，Huaqiao University，Quanzhou 362o21，China; 2.College of Chemical Engineering and Materials Sciences，Quanzhou Normal University，Quanzhou 362ooo，China）

Abstract：The eficacy of the C5-12 promoter in reducing or abolishing the immune response against ovalbumin （OVA） was explored through experiments such as determination of transgene expression，in vitro antigen presentation，and the clearance of transduced positive cells.The experimental results showed that in mouse myoblasts C2Cl2，the C5-12 promoter had no impact on the expresson of OVA，while in mouse bone marrow dendritic cells JAWS I ，the C5-12 promoter significantly inhibited both the expression of OVA and the presentation of OVA antigenic peptides. After muscle administration，the C5-12 promoter could maintain the continuous high expression of OVA in muscle tissues，the humoral immunity remained at a relatively low level， the immune response in muscle tissues was relatively mild，and the clearance of transduced positive cells was significantly reduced.

Keywords：C5-12 promoter；recombinant adeno-associated virus （rAAV）；immune response； transgene;ovalbumin （OVA）

在传染性疾病的防治领域，骨骼肌已成为外源性生产、分泌相关治疗药物极具吸引力的靶向组织之二[1]。（剩余13925字）