欧洲小叶椴体细胞胚胎发生及植株再生体系建立

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中图分类号：S722;S792.36 文献标志码：A 文章编号：1000-2006（2025）04-0079-09

Plant regeneration through somatic embryogenesis in Tilia cordata

LI Yin1²，YU Sinong³，YAN Lingjun²，WANG Huanli²*，TANG Shijie 1，2 *

（1.Nanjing Universityof ChineseMedicine，Nanjing 21OO23，China；2.Instituteof Botany，Jiangsu Provinceand Chinese Academy of Sciences （Nanjing Botanical Garden Mem.Sun Yat-Sen），Jiangsu Key Laboratory for Conservation andUtilizationof Plant Resources，Nanjing 210014，China；3.Yancheng Forest Farm，Yancheng 224136，China）

Abstract：【Objective】Thisstudyaims todevelopacompletesomaticembryogenesissystem forTiliacordataby systematicallyoptimizing theculture medium composition to identifyoptimal conditions for somaticembryo inductionand subsequent plant regeneration.【Method】Immature zygotic embryos of T cordata wereused asexplants.The effects of diffrentconcentrationsandcombinationsofplantgrowthregulators2，4-dichlorophenoxyaceticacid（2，4-Dand 6-benzylaminopurine（6-BA）oncallusinduction，proliferation，and somatic embryo induction eficiency were systematicallyinvestigated.Additionally，theimpactsofdiferentbasalmedia，indole-3-butyricacid（IBA） concentrations，andactivated charcoal suppementationon somaticembryogerminationand plant regeneration were evaluated.【Result】Optimal callus induction was achieved at a concentration of 3.O mg/L 2，4 -D combined with 0.2 mg/L6-BA，yielding an induction efficiency of 64.25% .Subsequent proliferation of embryogenic callus demonstrated maximal growth potential under identicalhormonal conditions （3.0mg/L2，4-D+O.2 mg/L 6-BA），as evidenced byan exceptional proliferation coefficient of 1063.72% .Forsomatic embryo induction，the same hormonal formulation（3.0 mg/L 2，4-D+0.2 mg/L 6-BA） proved most effective，generating somatic embryos at a frequency of 46.41% . Complete plantregenerationwassuccesfullyaccomplished throughtransfering of developed somatic embryos toMSbasal medium 收稿日期Received：2024-11-20 修回日期Accepted：2025-03-12 supplemented with 0.5mg/L IBA in the absence of activated charcoal，with all regenerated plants exhibiting normal morphological characteristics.【Conclusion】In this study，a complete somatic embryogenesis system of T cordata was initiallyestablished，providingabasis forsubsequentestablishmentofaneficientpropagationandgenetictransformation system for Tilia.

Keywords：Tilia cordata；embryogenic callus；somatic embryo；somatic embryo regeneratio

欧洲小叶椴（Tiliacordata）属于锦葵科（Malvaceae）椴树属（Tilia），为落叶乔木，原产自欧洲，具有良好的观赏价值和较强的环境适应性。（剩余17741字）