平安竹缩短节间形成相关转录因子PjGRAS56功能研究

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中图分类号：S795；Q781 文献标志码：A 文章编号：1000-2006（2025）04-0012-11

Abstract：【Objective】Internode length isa crucial trait forbamboo industrial utilization.Pseudosasa japonica var. tsutsumianaisastabledwarfmutant of P . japonica，characterizedbyshortened，flask-shaped internodes，makingitan idealmaterialforstudying internode growth inbamboos.PreliminarystudiesidentifiedtheGRAS transcription factor PjGRAS56 as a key candidate gene responsible for the shortened internode formation in P . japonica var. tsutsumianais. This studyaims to elucidate the potential roleofPjGRAS56 inbamboo internodedevelopment.【Method】Weemployed bioinformatics toanalyze the sequencefeatures ofPjGRAS56，confocal laser scanning microscopy toobserve its subcelularlocalzation，ayeast tanscritionsystemtoassess itstransciptionalactivationactivity，transgenictechques in rice and Arabidopsis to analyze phenotypes of PjGRAS56-overexpressing plants，transcriptome sequencing andqRTPCR to examine gene expression changes，andhand-sectioning to measure internode cellength.【Result】PjGRAS56 localized tothe nucleusand cytoplasm，andlacked transcriptionalactivationactivity.It wassignificantlydown-regulated in the division zone of P .japonica var. tsutsumianais internodes.Overexpression of PjGRAS56 in rice resulted in dwarfism，shortened internodes，reducedtillering，shorter paniclesandawns，fewer grains，andreduced leaf angle; overexpressionof itinArabidopsisledtosmallerleavesandshorterflowerstalks.Althoughthecellengthininternodesof

PjGRAS56-overexpresing plants was similar to controls，thecell number wassignificantlyreduced. Transcriptome sequencinganddiferential gene expresionanalysisrevealedsignificant down-regulationofcelldivision-relatedgenes in PjGRAS56-overexpressing plants.【Conclusion】PjGRAS56isaputative negativeregulatorof internode growth inbamboo. Keywords：Pseudosasa japonica var.tsutsumiana; internode length； intercalary meristem；division zone;PjGRAS56

竹材是支撑“以竹代塑”国家行动的重要基础。（剩余19432字）