基于网络药理学及分子对接分析《石室秘录》解湿汤治疗痛风性关节炎作用机制

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[关键词］痛风性关节炎；解湿汤；网络药理学；分子对接；作用机制；抗炎；调节代谢[中图分类号］R285 ［文献标志码］A [文章编号]0256-7415（2025）17-0007-08DOI：10.13457/j.cnki.jncm.2025.17.002

Analysis of Mechanism of Jieshi Decoction from Shi Shi Mi Lu in Treating Gouty Arthritis Based on Network Pharmacology and Molecular Docking

WANG Chenxin1.2，QI Airong1.2 1.TheFourth ClinicalMedicalColegeofGuangzhouUniversityofChineseMedicine，ShenzhenGuangdong518o33，China；2.Senzhn Traditional ChineseMedicineHospital，ShenzhenGuangdong518O33，China

Abstract：Objective：To analyze the mechanism of Jieshi Decoction in treating gouty arthritis（GA）using network pharmacology and molecular docking.Methods：Active components and targets of Jieshi Decoction were retrieved from TCMSP and BATMAN-TCM databases.GA-related targets werecollcted from GeneCards，CTD，and OMIM databases.Venny platform identified overlapping targets.STRING database constructed a protein-protein interaction（PPI）network.GO and KEGG enrichment analyses were performedusing R software，with molecular docking validationvia AutoDock Vina.Results：A total of 59activecomponentsand295overlapping targets were identified.Core components included quercetin，styrene，cinnamic acid，ethylcinnamate，trans-cinnamic acid，and anethole.PPIanalysis revealed AKT1，INS，TNF，IL6，TP53，and ALBas key targets.GO enrichment highlighted biological proceses including response to xenobioticstimulusandresponse to nutrientlevels.KEGGanalysis identified 197signaling pathways，predominantly enriched inthe IL-17 and AGE-RAGE signaling pathways.Moleculardocking confirmed strong binding afinity betweencore components（quercetin，styrene）and key targets （TNF，INS）. Conclusion：Jieshi Decoction exerts anti-inflammatory，metabolic regulatory，and microenvironment-modulating effcts against GA byregulating targets likeAKT1and TNF throughactive components like quercetinand styrene， primarily via IL-17 and AGE-RAGE signaling pathways.

Keywords：Gouty arthritis；Jieshi Decoction；Network pharmacology；Molecular docking；Mechanism；Antiinflammatory；Metabolic regulation

痛风性关节炎（GA）是由嘌呤代谢紊乱引起的炎症性风湿病，以关节及周围组织反复发作性红肿、疼痛为主要临床表现。（剩余10284字）