基于香豆素 ⋅Cd2+ 配合物的 Cu2+ 荧光探针构建及其应用
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Construction and Application A Cu2+ Fluorescent Probe Based on Coumarin- Cd2+ Complex
XIAOJiayang'，ZHUXinyue’，GUOXiaoyun’，YANGFan1，YANGTairan²， CHENRui²，GAO Yunl*，GAO Yan1* （1. Engineering，UniversityScienceandTechnologyLiaoning，Anshan14o51，China; 2.YingkouFoodandDrugTestingCenter，Yingkou115oO3，China） * Corresponding Authors，E-mail：yuner9111@163.com；gys20080901@163.com
Abstract： In this paper，the ligand ABX was prepared by condensation reaction coumarinaldehyde and pyrene hydrazone.The probe hascompletelydiferent UVandfluorescence responses towards Cdand Cu，respectively.Competitive experiments showed that the presenceCu²+ interferes with the Cd+rcognition performance byprobe ABX， and the substitution reaction between Cu2+ andABX-Cd²produceed a more stable ABX- Cu2+ complex.Therefore，the recognition performance ABX-Cd² complex for Cu2+ was investigated. The results showed that the probe ABX-Cd2+ could ientifyCu2 with high selectivity and sensitivity，and the fluorescence responseeficiency was significantlyimproved from 68. 71% to 96. 25% compared with that Cu2+ directlyidentifiedbyABX.The“test paper” theloaded probe ABX-Cdwas orange-red under ultraviolet light，and then the fluorescence was graduallyquenched after being immersed in Cu2+ ，so it was successfully applied to the semi-quantitative detection Cu2+ in tapwater and industrial wastewater.Inaddition，theleters writen withABXsolutiononthesilicagel wereyellowish greenunderultraviolet light and then became orange-red after applying Cd2+ ，and the fluorescence was completely quenched after continuing to apply Cu2+ ，which was suitable for application in the field encryptionand anti-counterfeiting ink.Most importantly， The probe ABX- Cd2+ has also been successfully applied to the bioimaging studies intracellular Cu2+ ：
Key words： fluorescent probe；coumarin; Cd2+ complex; Cu2+ ； industrial wastewater； fluorescent anti-counterfeiting;bioimaging
1引言
铜离子是人体必需的微量元素之一，它可参与蛋白质、DNA和RNA的合成，在维持人体正常生理功能、促进生长发育、维持神经系统健康、促进血红蛋白生成、调节免疫力方面发挥着重要作用。（剩余18587字）