菊花脑响应干旱胁迫的转录组学分析

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中图分类号：Q943.2 文献标志码：A

文章编号：1672-7010（2025）04-0063-11

Transcriptome analysis of Chrysanthemum nankingense in response to drought stress

WANG Tao ，WANG Wanyi，SHAO Qian，BI Shuyan，HAN Qiankun ，FAN Xianglong'，REN Hui'， ZHANG Xiaolong

（1.Department of Biology and Food Engineering，Bozhou University，Bozhou 2368Oo，China; 2.Anhui Engineering Research Center for the Development and Application of Functional Blended Liquor，Bozhou 2368OO，China；3.Bozhou Key Laboratory of Biosynthesis of Effctive Ingredients from Medicinal Plants，Bozhou 236800，China）

Abstract： Drought stress severely constrains the growth of Chrysanthemum nankingense （C. nankingense）， and elucidating its drought tolerance mechanisms holds significant implications for chrysanthemum breeding. Using C . nankingense 10-leaf-stage seedlings subjected to 40% PEG-6OoO-simulated drought，this study investigates molecular regulatory networks via transcriptome sequencing.Results identify 848 diferentially expressed genes（DEGs），with 484 significantly up-regulated and 364 down-regulated.Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analyses demonstrate DEG involvement in abscisic acid signaling，dehydration response，reactive oxygen species scavenging，and secondary metabolite synthesis.Key lignin biosynthesis genes（ PAL ，F5H，CST，CCoAOMT，CAD）exhibit markedly elevated expression under drought，correlating with 1.98- fold and 1.38- fold increases in lignin content in apical and basal stems versus controls.These findings suggest enhanced lignification constitutes a critical adaptive strategy to water deficit in C . nankingense.This research systematically reveals a multi-pathway coordinated molecular framework for drought response in c . nankingense，providing a theoretical basis for drought-tolerance gene mining and molecular breeding.

Key words： Chrysanthemum nankingense; drought stress； transcriptome; lignin

干旱胁迫作为影响植物生长发育的主要非生物胁迫因子，在气候变暖、人口增长和淡水短缺的作用下，农作物与观赏植物的产量劣化及其品质会受影响。（剩余9901字）