电针干预改善大脑中动脉栓塞大鼠脑部缺血机制的研究

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Electroacupunctureinterventiononmechanismofcerebralischemiainratswithmiddlecerebralarteryoccusion （MCAO）

YANG Yunrongl，FENG Hui’，GUAN Huazong²

'RehabilitationDeparment，uuiCentralHospital，anghai，hina;Depatmentfrevntieedicine，aa Hospital Afiliated to Shanghai UniversityofTraditional Chinese Medicine，Shanghai2ooo5o，China.

[Abstract]Objective：ToobservetheeffectofelectroacupunctureontheexpressionofHtrA1inastrocyteswithinthe hippocampaltissueofratswithacutecerebralinfarction.Methods：Twenty-fourmaleSDratswereselectedandrandomly divided intothesham-operation，model，andelectroacupuncturegroups，with8ratsineachgroup.Themodel and electroacupuncturegroupsunderwentrightmidlecerebralarteryoclusion（MCAO）modelingviatheintraluminal filament occlusionmethod.Thesham-operationgroupreceivedidenticalsurgical exposurewithout filamentinsertion.Twenty-fourhours aftersuccesful modeling，ratsintheelectroacupuncturegroupreceivedelectroacupuncture treatmentatBaihui（GV2o）and Shenting（GV24）using dense-disperse waveswithalternating frequencies of2/15 Hz andacurrent intensityof 1.5–2 mA. Cerebralblodflowchangesastrocyteexpressionandactivationstatus，HrAlmRNAlevels，HtrAlproteinexpressonlevels， and TNF- α ，IL- 1β ，and IL-6 levels of three groups were measured and compared seven days after the operation.Results： WesternblotingshowedthatHtrA1expressioninthemodelgroupwassignificantlyincreasedcomparedtothesham-operation and electroacupuncture groups（both P<0.05 ）.Quantitative real-time PCR （qRT-PCR）showed that HtrA1 mRNA expression level in the electroacupuncture group was significantly lower than that in the model group（ P<0.05 ）.ELISA showed that the model group had the elevated levels of TNF- α ，IL- 1β ，and IL-6 compared to the sham-operation group （all P<0.05 ），while the electroacupuncture group had the reduced levels of TNF- α，lL-1lβ ，and IL-6 compared to the model group（all P<0.05 ）. Conclusions：lectroacupuncturecanleviateijuryproerologicalfuctioancerebralbloodflondpst inflammatoryresponseandthemechanismmaybeassociatedwiththeinibitionof HtrA1proteinexpressionandsuppressonof astrocyte polarization.

[Keywords] Electroacupuncture;Astrocytes;Cerebral ischemia reperfusion;Cerebral infarction

脑卒中已成为全球范围内造成残疾甚至死亡的主要疾病之一[1]，其中，缺血性脑卒中（cerebral ischemicstroke，CIS）是最常见的类型，也是最具神经破坏性的疾病之一[2]。（剩余7180字）