基于茎尖脱毒的宁夏枸杞无病毒种苗制备技术研究

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中图分类号： S567.1+9 文献标识码：A 文章编号：1002-204X（2025）07-0022-06

doi：10.3969/j.issn.1002-204x.2025.07.004

AStudy on Preparation TechnologyofNingxia Lycium barbarum Virus-Free Seedlings Based on Shoot Tip Detoxification

Zheng Pinjie，Wang Yuhan，Shu Zhenyu， Zhang Xin* （School of Biological Science and Engineering，North Minzu University，Yinchuan，Ningxia 750002）

Abstract [Objective]To address the issue of germplasm degradation of Ningxia Lycium barbarum caused by viral diseases，and to establishanefficient stem tip virus-freetechnology system，providing technological support for the large-scale production of virus-free seedlings.[Methods] Using the stem tips of "Ningqi No.1" as explants，and taking survivalrate，germinationrate，rediffrentiationrateanddetoxificationrateasevaluation indicators，thehormoneratio of 6-BA （0.05-0.5 mg. L-1 ）andNAA （0.01-0.2mg·L -1 ）inMS medium wassystematically optimized to screen the optimal stem tip length.And the detoxificationefects of each treatment were detected byELISA.[Result]When the medium was a combination of 0.2 mg·L-1 6- BA + 0.05 mg* L-1 NAA，the induction rate of tissue culture seedlings was the highest， reaching 73.33%±6.7% ;When the concentration of NAA was 0.1mg⋅L-1 ，thedifferentiation rate reached （204 80%±6.7% ，and the root development was optimal （ P<0.05 ）. The length of the stem tip was significantly negatively correlated with the detoxification efficiency （ r2=0.904 3 3）.Stemtips withalength of O.21-O.3Ommhadbothahigh survival rate 62.2%±3.8% ）anda high detoxification effect （82.2%±3.8% ）.[Conclusion] By optimizing the hormone ratioandthe length of thestem tip，The virus-free seedling preparation technologyestablishedforNingxia Lycium barbarum has a virus removal rate of over 82% and a transplanting survival rate of over 65% ， providing a standardized solution for the prevention and control of Lycium barbarum viruses and the improvement of their quality. Key Words Ningxia Lycium barbarum; Preparation of virus-free seedlings;Apical meristem; Tissue culture; Seedling production

丁夏枸杞（LyciumbarbarumL.）作为我国四北地区重要的药食同源经济作物，其产业发展对区域经济和生态建设具有重要意义[-2]。（剩余8075字）