感病对滇重楼根茎中甾体皂苷积累的影响

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中图分类号：S567.239.01 文献标志码：A 文章编号：1004-390X（2025）03-0117-09

Abstract： [Purpose] To compare the differences of steroidal saponins content in healthy and susceptible rhizomes of Paris polyphylla var. yunnanensis，analyzing the effects of infection on the steroidal saponins content in rhizomes of P . polyphylla var. yunnanensis. [Methods] The contents of eight steroidal saponins （I，II，II，V，VI， VII，Hand slender dioscin） in the rhizomes of121 healthy and 106 susceptible P . polyphylla var. yunnanensis were determined by high performance liquid chromatography，and the independent sample t⋅ -testanalysis and orthogonal partial least squares discriminant analysis （OPLS-DA） were used to compare the diferences of steroidal saponins content in the rhizomes of healthy and susceptible plants. [Results] The total content of eight steroidal saponins in the rhizome of P polyphylla var. yunnanensis was mainly composed of polyphyllin I， I and VIl， accounting for about 71% ; polyphyllin II，V，VI， H and slender dioscin were supplemented， accounting for about 29% .The total content of eight saponins in the rhizomes of susceptible P polyphylla var. yunnanensis from 12 origins Was higher than that of healthy plants. Except for Taian Township and Ludian Township of Yulong County， the other 10 origins showed significant diferences between susceptible and healthy plants，of which seven origins showed an extremely significant differences. Based on the OPLS-DA analysis of eight saponin contents， the differential markers of healthy and susceptible P . polyphylla var. yunnanensis were polyphyllin I， II and VII. [Conclusion] Steroidal saponins content in the rhizomes of susceptible P ：polyphylla var. yunnanensis is generally higher than that of healthy plant. When the plants of P . polyphylla var. yunnanensis are subjected to biological stress， they may promote the synthesis and accumulation of steroidal saponins in rhizomes.

Keywords： Paris polyphylla var. yunnanensis （Franch.） Hand.-Mazz.; infection; saponin accumulation;high performance liquid chromatography; content differences

滇重楼[Parispolyphyllavar.yunnanensis（Fra-nch.）Hand.-Mazz.]为百合科（Liliaceae）重楼属（Pa-ris）多年生草本植物，是中国众多著名中成药的主要原料，具有清热解毒、消肿止痛、凉肝定惊的功效，适用于疗疮痈肿、咽喉肿痛、蛇虫咬伤、跌打损伤、惊风抽搐等症[1。（剩余10960字）