重楼8-双分病毒1号的首次报道及其近全长基因组序列分析

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中图分类号：S435.672 文献标志码：A 文章编号：1004-390X（2025）03-0020-0

Abstract： [Purpose] To clarify the taxonomic status and occurrence of the new members of genus Deltapartitivirus in family Partitivridae discovered on Paris yunnanensis. [Methods] High-throughput sequencing （HTS） was used to detect the P .yunnanensis leaf samples with virus-disease-like symptoms collected in 2020 from Lijiang City， Yunnan Province， obtaining the nearly complete genome sequence of a novel deltapartitivirus-like in genus Deltapartitivirus and temporarily designated as Paris deltapartitivirus 1 （ParDPV1）. RT-PCR technology Was used to amplify ParDPV1， and its classification was Validated based on nucleotide and amino acid sequence identity， as well as phylogenetic analyses of CP and RdRp.By testing 798 samples collcted in 2020，2023 and 2024，the occurrence of ParDPV1 in the field was assessed. [Results] The nearly complete genome sequences of ParDPV1， comprising 1570 bp （dsRNA1） and 1522 bp （dsRNA2）， were obtained from P yunnanensis leaves exhibiting mild motling symptoms. Sequence analysis showed that these two segments encoded the RdRp and CP of the virus， and the amino acid identities of RdRp and CP with members of the genus Deltapartitivirus were 34.80%6-62.18% and 17.51%-29.19% ，respectively，which were significantly lower than the taxonomic thresholds for this genus（ ⩽90% of RdRp， and ⩽80% of CP）. The phylogenetic tree constructed based on RdRp and CP amino acid sequences showed that ParDPV1 was clustered with members of the genus Deltapartitivirus. RT-PCR screening of 798 samples revealed that ParDPV1 was detected only in three plants exhibited no obvious symptoms or mild motling. [Conclusion] ParDPV1 is an new member in genus Deltapartitivirus， occurring only sporadically in the field and causing no or only mild symptoms in infected plants. It is proposed that “Paris deltapartitivirus 1∘ （ParDPV1） as its generic name， and Deltapartivirus paris of its species name.

Keywords： Paris yunnanensis; virus disease; Paris deltapartitivirus 1; sequence analysis; species identification

重楼属（Paris）植物为黑药花科（Melanthiaceae）多年生草本植物[，普遍含有甾体皂苷类、黄酮类、三萜类、多糖类等化学成分，具有抗肿瘤、抑菌、抗氧化、镇痛、正血等药理活性以及清热解毒、消肿止痛、凉肝定惊等功效1，是中国汉医药及藏、傣、维吾尔、蒙古族等民族医药中常用的药材，也是云南白药、季德胜蛇药、宫血宁胶囊、金复康口服液、楼莲胶囊等80余种重要中成药的主要原料[2，在中国中医药大健康产业中占有重要地位[3。（剩余12587字）