高黎贡山地区尤犀金龟属昆虫物种分子鉴定

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中图分类号：S718.7 文献标志码：A 文章编号：1004-390X（2025）03-0028-08

Molecular Identification of Eupatorus spp. （Insecta） in the Gaoligong Mountain Region

FU Zhonghong¹，YIN Jing¹，DUANKeyi’，LUFei¹，YI Chuanhui1²，LIU Qing³，HE Qiuju14

（1. College ofForestry，Southwest Forestry University，Kunming 650224， China; 2.Yunan Academy ofBiodiversity. Southwest Forestry University， Kunming 650224， China; 3. Research Institute of Gaoligong Mountain， KeyLaboratory of Conservationand Utilization of Insect Resources in Western Yunnan，Baoshan University，Baoshan 6780oo， China; 4.KeyLaboratory ofForestDisasterWarning and Control of Yunnan Province， Southwest Forestry University， Kunming 650224， China）

Abstract： [Purpose] To explore the species definition of Eupatorus Burmeister，1847 through the application of mitochondrial DNA barcoding technology，resolving the challenges associated with the morphological identification of species within this genus.[Methods] Employing mitochondrial coxl and cox2 gene sequence datasets， the molecular species of the three morphospecies were defined by the Automatic Barcode Gap Discovery （ABGD） and Bayesian Poisson Tree Processes （bPTP） methods. Subsequently， the obtained results were compared with those of morphological identification. [Results] The definition results of the coxl dataset using the ABGD method were congruent with the morphological identification outcomes.However， disparities were observed between the cox2 dataset and the morphological results. In the bPTP method， the definition results of the two datasets were significantly higher than those of morphological identification， demonstrating varying degrees of over-division. [Conclusion] The coxl gene is more suitable for use as a DNA barcode in the identification of Eupatorus insects.The results of the coxl dataset defined by the ABGD method are consistent with morphological identification. By integrating molecular definition and morphological characterization， the efficiency and accuracy of species identification can be substantially enhanced.

Keywords： Eupatorus; classfication; species molecular identification; cox1; Automatic Barcode Gap Discovery （ABGD）

尤犀金龟属（EupatorusBurmeister，1847）隶属鞘翅目（Coleoptera）金龟科（Scarabaeidae）犀金龟亚科（Dynastinae），目前已知7种，分布于中国、印度、缅甸、老挝、越南和泰国[1-3]。（剩余12674字）