滇龙胆PGIP基因家族全基因组鉴定及表达模式

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中图分类号：S567.239.01 文献标志码：A 文章编号：1004-390X（2025）03-0097-11

Abstract： [Purpose] To explore the potential function of the polygalacturonase-inhibiting protein （PGIP） gene in the corolla temperature sensing movement of Gentiana rigescens. [Methods] Based on the genome-wide and transcriptome data of G .rigescens，bioinformatics methods were used to analyze the physicochemical properties， gene structure， phylogenetic relationships， conserved structural domains， cis-acting elements and expression patterns of the PGIP gene family. [Results] The GrPGIP gene family contained 10 members， distributing unevenly on the chromosomes 1， 2， 4， 5，and 9. The GrPGIP genes all contained exons. GrPGIP were all hydrophilic proteins， and subcellular localisation predicted that they were located predominantly in the cell wall. The promoter regions of most GrPGIP gene family members contained light-responsive elements and hormone-responsive elements. Colinearity analysis showed that the PGIP gene family was conserved in dicotyledonous plants， and GO enrichment analysis indicated that the GrPGIP genes might play important roles in the cll wall. 10GrPGIP genes were differentially expressed during the corolla temperature sensing movement in G. rigescens， which suggested that the GrPGIP genes might be involved in the corolla movement. [Conclusion] The results of the study preliminarily elucidate the expression pattern of GrPGIP （20 during the corolla temperature sensing movement， laying the foundation for further research on the function of GrPGIP in the corolla temperature sensing movement in G. rigescens.

Keywords： Gentiana rigescens; temperature sensing movement; polygalacturonase-inhibiting protein （PGIP）; cell wall

滇龙胆（Gentiana rigescensFranch），俗称坚龙胆、龙胆草、兰草根等，为龙胆科（Gentianace-ae）龙胆属（Gentiana）多年生草本植物[1，生长在海拔 1100～3000m 的山丘草地、灌木丛、乔木林下以及峡谷地带。（剩余17314字）