大豆谷氨酸受体通道蛋白基因家族鉴定与表达分析

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Abstract：Glutamate receptor like channels （GLRs）area type of Ca2+ channel proteins involved in cellular metabolism，seed germination，plant growth and development，as wellas long-distance signal transmission. In this study，a total of 29 soybean GLR members were identified through a comprehensive proteomic identification. Their phylogenetic relationship suggests that these GLR genes can be classified into three subfamilies，and members within the same subfamily exhibits conserved gene structure and motif compositions. Cis-acting elements analysis indicates that the promoter regions of these genes contain a number of regulatory elements related to hormones，stress，light，and plant growth and development.The analysis of tissue-specific and stress-induced transcriptional profiles showed that GLR genes were highly expressed across various tissues and involved in salt and alkaline stress.GmGLRs including GmGLR3.13，GmGLR3.15，GmGLR4.8，GmGLR4.9 and GmGLR4.l0 ，exhibited significant transcriptional variations under salt and alkaline stress using qRT-PCR analysis，suggesting their important roles in stress response. In summary，the results of this study provide an important theoretical basis for further investigating therole of GLR genes responsible to stress challnges in soybean.

Key words： soybean; GLR;whole genome identification;saline and alkaline stress

土壤盐碱化导致土壤渗透压上升，作物根系无法从土壤中吸收水和养分，进而造成水分流失，导致作物脱水、枯萎、甚至死亡，最终影响产量。（剩余21677字）