AT11与GS3互作调控水稻耐碱性

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Abstract： （Oryza sativa L.），a critical cereal crop sustaining global food security，faces severe yield limitations due to soil alkalinity. Enhancing the salt-alkali tolerance rice varieties represents apivotal strategy for boosting overallproduction and ensuring food security through expandingcultivable land in saline-alkaline environments.In this study，a mutant M23 with significantly reduced alkaline tolerance was identified from the γ -ray induced mutation library the Japonica rice variety Sasanishiki（WT）.Following a map-based cloning strategy，the target gene wasallocated in an 87.7kb region between markers P2 and P3 on chromosome 11， containing nine open reading frames （ORFs）.Sequence comparison revealed a four base pair deletion in the third exon ORF9 in the mutant，leading to premature termination.Therefore，ORF9 is hypothesized to be the strong candidate gene，designated Alkaline Tolerance ll （ATll），which encodes a putative peptide transporter （PTR）. CRISPR/Cas9 knockout lines ATll showed decreased alkaline tolerance，whereas its overexpression significantly enhanced alkaline tolerance.ATll is expressed in all tissues with the highest expresion in the panicle in rice. Yeast two-hybrid （Y2H） assay and Immunoprecipitation assay revealed that AT11 can interact with GS3，the heterotrimeric G-protein γ subunit in rice.Bimolecular fluorescence complementation （BiFC）

experiments pointed out their interaction on the plasma membrane.Through transcriptome sequencing analysis， the differentially expressed genes between WTand ATll knockout lines were significantly enriched in pigment binding and photosynthesis.Yield testingunder field conditions at pH=9.0 showed that ATll overexpression significantly increased yield production，demonstrating thatATllcan enhance the productivity riceon salinealkaline soils，with a potential for rice breeding with salt alkaline resistance.

Keywords： rice;alkaline tolerance;yield;ATll;GS3

气候变化对农业生产造成了负面影响，全球变暖限制了淡水资源的可用性。（剩余14420字）