中油蟠9号’桃套袋果实花色苷合成的转录组-代谢组联合分析

打开文本图片集

Abstract：Bagging-produced flat peaches have gained increasing consumer preference due to their reduced fruit cracking，enhanced visual appeal，and golden to slightly reddish fruit surface.Thecultivar‘Zhongyoupan g， shows a distinctive reddish coloration under bagging conditions，atributed to anthocyanin synthesis.This characteristic makes it particularlysuitable for baggngcultivation，aligning with consumer preferences for optimalcolor and appearance.In this study，we investigated the exocarp of bagged fruits of'Zhongyoupan 9'at 89 and102 days after blooming（DAFB），through an integrated approach combining transcriptomic， metabolomic，andbi-histological analyses to elucidate thecoloration mechanism.Transcriptomeanalysis identified 2652 significant diffrential genes，including16 genesassociated with anthocyanin biosynthesis （ PpPAL ，PpC4H，Pp4CLs，PpCHSs，PpCHIs，PpF3H，PpF3'H，PpDFR，PpANS，PpUFGT，PpGST），56 specifically up-regulated transcription factors，and phytoreceptors PpCRY3 ，PpUVR8.3. These genes were upregulated and expressed correlating with anthocyanin content.Metabolomic profiling using high performance liquid tandem massspectrometry detected112significantly diferential metabolites，with cyanidin-3，5-Odiglucoside showing the most substantial up-regulation （ Log2FC=16.8 ）.Thismetabolite was identified as the primary contributorto theredcoloration in the exocarp after bagging.Integrative analysis of transcriptomic and metabolomics data，combined with transcription factor profiling，revealed that PpBL and PpNACI up-regulated and activated the anthocyanin regulatory gene PpMYBI0.I .This activation promoted the catalytic synthesis of cyanidin-3，5-O-diglucoside by anthocyanin structural genes under baggingconditions，ultimatelyresulting in the characteristicred coloration of‘Zhongyoupan 9'.These findings advance our understanding of anthocyanin accumulation paterns inbagged peach fruits and provide a theoretical basis for selecting optimal peach varieties for bagging cultivation practices.

Key words：bagged；‘Zhongyoupan9’；anthocyanin；transcriptomics-metabolomicscombined analysis; qRT-PCR

桃[Prunuspersica（L.）Batsch]是世界温带地区栽培的重要果树之一，起源于中国，是我国重要落叶果树。（剩余16671字）