小麦籽粒色泽性状的全基因组关联分析及候选基因挖掘

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Abstract：Wheat kernel color related traits L* ， a* ，and b* are important indicators of wheat quality. To elucidate the genetic architecture underlying these traits，we conducted a comprehensive genome-wide association study（GWAS）using 243 wheat accessons from Huang-huai wheat region.Phenotypic evaluations were performed in four environments，complemented by genotyping using the wheat 66OK SNP assay.We identified 785 significant SNP markers associated with kernel color traits，collectively explaining 11.4%-23.4% phenotypic variation.Marker-trait asociations exhibited distinct chromosomal distributions：SNPsassociated with L* are mainly located on chromosomes 1A，1D，2B，3D，7A and 7D；SNPs associated with a* are primarily distributed on chromosome 2A，2B，2D，4B，5B，5D，7A and7D；SNPs associated with b* （204号 are predominantly distributed on chromosome 2B，5B，5D，6D，7A，7B and 7D.Notably，51 SNPs showed pleiotropic effects.Thirty-six candidate genes related to the kernel color traits were identified by gene annotation，of which eight exhibited kernel-specific expression pattrns.Polymorphism analysis revealed significant associations between target traits and two key genes，Pinb（TraesCS5Do2G004300）

and UDP-glucose/GDP-mannose dehydrogenase gene TraesCS5B02G399800. Haplotype analysisof TraesCS5B02G399800 identified distinct allelic variants significantly associated with b* .Therefore，these findings provide valuable genomic resources for marker-asssted selection and genomic prediction strategies for wheat kernel color traits.

Keywords：wheat;kernelcolor;GWAS

小麦籽粒色泽是小麦品质的重要指标之一。（剩余17684字）