OsELF3-1与OsARID3互作调控水稻根部性状

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Abstract：The root system is a criticalcomponent of rice plant，playing essential roles in plant fixation， wateruptake，andnutrient acquisition.In this study，weobserved that therootsystemof theoself3-1 mutant was significantlyshortercompared to thewild type.To investigate the genetic basis of this trait，the mutant was crossed with the wild-type cultivar Sasaishiki to generate an F2 population. Genetic analysis revealed that the mutant phenotype is controlled by a recessive single gene.Through map-based cloning，OsELF3-1 was located ina 50.9kb interval on chromosome 6，which contains four open reading frames （ORFs）.Sequence analysis identifieda7-base pair deletion in the second exonof ORF4（OsELF3-1），resulting in frameshift mutation and premature termination.OsELF3-1 was hypothesized as the causal gene.Knockout mutants of OsELF3-1 exhibitedsignificantlyshorterrootsthewild type Sasaishiki，therebyvalidating theroleof OsELF3-1 in regulating root length.To further elucidate theregulatory networkofOsELF3-1，weemployedayeast twohybrid screening and identified OsARID3 as an interacting protein.OsARID3 contains several functional domains，includinganARIDdomain，an a -crystallin/Hspdomain，aHsP20domain，andapotassiumdependent sodium/calcium ion exchange domain.Investigationof OsARID3-RNAi mutants revealed showed that their mutants alsodisplayed significantly shorterroots thanwildtypeSasaishiki，suggesting thatOsARID3 is involved in the regulation ofroot length.Analysis of natural variation in OsARID3using 3K rice genome sequencing data identified15haplotypes，with distinct differentiationbetween indicaand japonicasubspecies.Notably，japonica ricepredominantlyharbored haplotypesI，IVandVI.Insummary，thisstudy found that OsELF3-1and OsARID3 interact to regulate riceroot development.These findings provide a theoretical basis and valuable germplasmresources for further exploration of the regulatory networks underlying root growth and development.

Key words： rice;root;OsELF3-1;OsARID3 ;map cloning

水稻是重要的粮食作物，为我国超过半数的人口提供主食。（剩余16383字）