心脏归巢肽功能化修饰的脂质体负载秋水仙碱对心肌梗死后心脏功能的影响

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Effects of Cardiac Homing Peptide-Modified Liposomes Loaded with Colchicine on Cardiac Function Following Myocardial Infarction

ZHANG Xing，SONG Jie，Dilare·Taiwaikuli，DING Huanhuan，CHEN Yemin，WANG Xiaoyan，JIAN Yi，LIANG Junqing， FAN Zhongxiong，ZHOU Xianhui （Department of Cardiac Pacing and Electrophysiology，The First Afliated Hospital of Xinjiang Medical University， Xinjiang Key Laboratory of Cardiac Electrophysiology and Remodeling，Urumqi 830o54，Xinjiang，China）

【Abstract】ObjectiveTo construct liposomes loaded withcolchicineandmodified withcardiac homing peptide（CCP）and investigatetheirfectsoncardiacfunctionafermyocardialinfarction（M）.MethdsCCPwerepreparedusingthethin-filmhydration method.ThemorphologyofCCPwascharacterizedbytransmissonelectronmicroscopyH9c2ratcardiomyocytehypoxiamodelswere establishedtoevaluatethco-loalzationofCPwithardioyoyes.Tecellviablitywasasessdusingthellountingit-8ssay. Cellaruptakeandco-lcalizionwereobseedbyconfocallasersaningmicopy.SDatseredvidedinto6groupsha，P， colchicine-loadedliposoes（C）colicine（COL），lposomes（L）andMTreatmentswereadministeredviatailveinictiofor4 weks.Echocardiogaphyasperfoedatwek4folowedbyhistopathologicaltaningofyocardialtisses.ResultsBothCCandCP exhibiteduniformsphericalmorphology.Theparticlesizeandpolydispersityindex（PD）ofCPwere（183.5±2.1）nmandO.1960.014， respectively，while CCP measured （232.2±8.0）nm and 0.299±O.O51.In vitro，CCP demonstrated higher cellular uptake than COL and CP， withconfocaliaingcofiingectiecardoyocygetingbardacoingpeptide.InioteCC，C，ndCOousod reducedolpstddeltdedolfiadoo revealedsignifcantlyateuatedmyocadialfrosisinthCCPgoup.Echocadiogaphyidicatedmarkediprovementinadacfuction parametersintheCCP，CP，andCOLgroupsversustheMgoup.ConclusionCCPeectivelytargethypoxiccardiomyocyes，viate myocardial fibrosis，and improve cardiac function post-MI，providing anovel therapeutic strategy for MI treatment.

【Keywords】 Colchicine;Liposome ; Myocardial infarction;Cardiac homing peptide

急性心肌梗死（acutemyocardialinfarction，AMI）是临床上常见的心血管疾病，具有高致残率和致死率。（剩余12622字）