综合生物信息技术及实验鉴定动脉粥样硬化斑块过程中泡沫细胞形成关键基因-CFP

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【Abstract】ObjectiveMacrophagesareessentialcellarcomponentsintheinflammatoryprocessAtherosclerosisisachronic inflamatoryposscodaedcllpooofosisowe reportsonthenalysisofcaracteisticgensintisproessMethodsSingle-celsequencingdatafatheroleroticplaquere analyzedtoidentifhelliolednddeterineeiranscriptoeharacteistics.GeneotoloyKyotocyopediaofead genomes nrichmentaalysis，BERSORTrandomforestandweightedcorelationetworkanalysisereapledtoatchsequengdata. Alldataeredownloadedfromthegeneexpresionomius.Teprotein-proteininteractionnetworkwasconstructedusingtheSTING database，andthehubgeneswerefurtheridentifiedusingthecytoHubbaalgoithminCytoscape.ROCanalysiswasusedtoevaluatethe diagnosticeffcacyofhubgenes.TeCBERSORTalgorihmwasusedtoanalyzetheinfilrationofimuecells，andtheSpeaanank corelationanalysisiRftareasusdtoexploeecoelationbetwnteidtfiedbiomarkersndteifiraingiecel. Finally，weconfidthexressonofthcentalgeeinfacllsyplasmidtransfetionResultsoughdiferentialexpeson analysis，7iteseoitillyxpredesredutductiolntalyiodateily relatedtotheimmuneresponse-regulatorysignalingpathwayCFPwasidentifiedasaubgneandverifieditheGSE28829dataset. Immunocyte infiltration analysis showed that CFP was positively correlated with CD8 + T cells，regulatoryTcells，resting dendritic cells， macrophageM2ndactivateddenditiccels.TheresultsofplasmidransfectionshowedtatCFwashighlyexpresedifoamcelshich wasconsistentwithoforatisanalysis.ConclusionThisstudyconfisthatCFmayplayakeyoleintheournceanddevelopnt fatherosclerotic plaques，and opens up newideas for further studyofthepathogenesis of atherosclerosis 【Keywords】Atherosclerosis;Macrophage;Bioinformatics;Plasmid transfectior

心血管疾病（cardiovasculardisease，CVD）是威胁人类身体健康和生命安全的主要疾病，而动脉粥样硬化性心血管疾病（atherosclerotic cardiovascular disease，ASCVD）在CVD中的占比也呈逐年升高趋势。（剩余10964字）