细胞外基质硬度对原代人前列腺癌细胞炎症因子 表达的影响

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[中图分类号] R737.25 [文献标志码] A

Influence of extracellular matrix stiffness on the expression of inflammatory factors in primary human prostate cancer cellsLI Ruizhi，MU Jie， ZHU Hai，LI Jing，WANG Dong（Institute of Chronic Diseases， Qingdao Medical College of Qingdao University，Qingdao 266071，China）

[ABSTRACT]ObjectiveTo investigate the influenceof extracelular matrix （ECM）stifnessontheexpressionof inflammatoryfactorsinprimary humanprostatecancercels.MethodsPrimary human prostatecancercellswereinoculated intoculture dishes pre-incubated with 0.1g/L ECM solution （group A） or 8g/L ECM solution （group B），and bright-field microscopy was usedto observe cellmorphology.Onday4ofculture，RT-qPCR and Western blot were used to measure therelative mRNA and proteinexpressionlevelsoftheinflammatoryfactorsC-Cmotifchemokineligand2（CCL），C-X-Cmotifchemokineligand10 （CXCL1O），intercelularadhesion molecule-1（ICAM1），andinterleukin-32（IL-32）incell.ResultsBright-fieldmicrosopy showedthatat24oursafterinoculation，thecellsingroupAgraduallyaggregatedandformedsht-likestructures，whilethose in groupBexhibited multilayeredcellaggregates.RT-qPCR showed thatcompared with groupA，group Bhadsignificantlyhigher relative mRNA expression levels of the inflammatory factors CCL2，CXCL10，ICAM1，and IL-32 （ t=5.30-16.23，P<0.0 5）. Western blot showed thatcompared with groupA，group Bhad significantly higher relative protein expresion levelsof ICAM1， IL-32，and CCL2 （t=2.78-14.72，P<0.05） ，while there was no significant difference in the relative protein expression level of CXCL10 between groups A and B（ P>0.05 .Conclusion Soft ECM substrate can promote the expression of the inflammatory factors CCL2，CXCL1o，ICAM1，and IL-32 in primary human prostate cancer cells.

[KEY WORDs]Prostatic neoplasms; Primary cellculture; Extracellular matrix; Hardness； Cytokines

前列腺癌在全球范围内的发病率及死亡率呈快速上升趋势。（剩余8657字）