瘤内Gemellahaemolysans对增强食管鳞状细胞癌荷瘤小鼠新辅助化学免疫治疗疗效的研究

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【中图分类号】R655.4 【文献标志码】A

【AbstractObective：TinvstigatehtherintratuoralGemellahemolysans（Gamolysans）enanesteficacyoadjuvant

chemoimmunotherapy（NACI） inesophageal squamous cellcarcinoma（ESCC）-bearing micetheunderlying mechanism.Methods：

Twenty-five 4-week-old female C57BL/6 mice were romized into five groups（ n=5 per group） ： negative control（NC） group，chemo

therapy group，G.haemolysans + chemotherapy group，NACI group， G.haemolysans + NACI group. A germ-free mouse model was

establishedineachgroupbyadministeringantibioticcocktailfor2weeks.OndayO，ECC-bearing mousemodelswerebuiltthrough

subcutanous inoculation ithermurine esophagealcancercelline AKRor G.haemolysans-AKRcels.Onday7whentumors

formedundertheskin，drugswereinjectedintotheabdomenthricewekly.Onday28whenthetreatmentswerefinished，themice

weresacrificed to harvest their tumors， then the tumors were fixed in 4% paraformaldehyde.We locatedquantifiedG.haemolysans

withinthetumorsthoughfuoresceceinsituhbridizatio，ndcharacterzedimunecellompositioninthetumorimmuneo

vironment（TIME）themicetreatedwithNACIthroughmultipleximmunohistochemistryflowcytometry.Results：G.haemolysans

significantly strengthened the efficacy NACI in ESCC（ P<0.05 ），whereas no chemosensitizing effects were observed on chemo

therapy in ESCC-bearing mice.G. haemolysans significantly enhanced natural killer（NK）cell infiltrationwithin the TIME（ P <0.01）.

Conclusion：G. haemolysans improves NACI efficacy in ESCCbearing micebyincreasingNKcell recruitmentin the TIME.

【Keywords】esophageal squamous cell carcinoma;Gemella haemolysans ;neoadjuvant chemoimmunotherapy

食管癌（esophagealcancer，EC）是全球范围内常见的恶性肿瘤之一，其发病率和死亡率分别占全球恶性肿瘤的第七位和第六位。（剩余13359字）