碳纤维基皮质醇电化学汗液选择电极的制备与表征

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中图分类号：TP212；R318.08 文献标志码：A 文章编号：1671-024X（2025）03-0012-11

Fabrication and characterization of cortisol-selective electrochemical sweat sensor basedoncarbonfibersubstrate

LIU Hao 1，2，3 ，LI Zhijie1，2，3，LUODan1，2.3，LI Jun1，2.3 （1.SchoolofTextileScienceandEnginering，Tiangong University，Tianjin3O387，China；2.Research CenterforFuture TextileTechnologyTiangongUnversityTianjinOO387，China;3.ChinaInsituteofSmart WearableElectroniciles， Tiangong University，Tianjin 300387，China）

Abstract：Todevelopahigh-stabilityand high-sensitivityprobe-freecortisolsensorforwearablesweat monitoring，acarbon fiber（CF）-based composite electrode system was designed.Achitosan （CS）-Prussian blue （PB）/CF substrate was constructed through PB electrodeposition and CS coating via vacuum filtration.Subsequently，a molecularly imprinted polymer （MIP） layer was prepared using 0 -phenylenediamine as the functional monomer，ultimately forminga probe-free MIP/CS-PB/CF electrode.The morphology，electrochemical performance，and flexibility of theelectrode were investigated.Resultsdemonstrated thatPBand CS synergisticallenhanced electrode conductivity，whilethe MIPlayer enabled efficient recognition.The sensor achievedadetection limitof O.235 nmol/L with a linear range from1 to 10∘nmol/L ，exhibiting strong anti-interference against steroid analogs and common metabolites （selectivity coefficient >2 ）. The electrode maintained stable performance with <1% resistance variation after1 2Oo bending cycles，demonstrated 3.01% relative standard deviation（RSD）in inter-batch repeatability，and showed only 2.4% signal attenuation over 7d.This electrode combines high sensitivity，selectivity，and mechanical stability，providing areliable technical solution for wearable health monitoring systems.

Key Words：carbon fiber;cortisol; molecularly imprinted polymer；sensor； electrochemical; sweat

皮质醇作为人体应激反应的关键生物标志物，其动态监测在健康管理、疾病诊断及压力评估中具有重要意义[1-2]。（剩余19440字）