高产 a -淀粉酶基因工程菌A8-1的发酵优化及应用
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中图分类号： TS4l+1 ；Q815
文献标识码：A
文章编号：1007-5119（2025）05-0077-10
Fermentation Optimization and Application of High-Yield α-amylase Genetically Engineered Bacterium Strain A8-1
ZHANG Zekun1， DUAN Xiaojiao1， YE Changwen²，AN Lu1， WU Shuangshuang1，LI Liqun1， XI Hongru²， HAO Jiel*， NIU Chenqi2* （1.InnerMongoliaKumingCigareteLimitedLiabilityCompanyHohotOoo，China;2.ZengzouTbaoReseachsite of China National Tobacco Corporation， Zhengzhou 45ooo1，China）
Abstract： In order to improve the a -amylase production activity of genetically engineered strain BAX-5/PT-17 SPo02amy（A8-1）， improveitsindustrialapplcabilityandapplicationeffectintobacoprocessng，aseresof parameters inthefermentationproce wereoptimizedthroughsinglefactortestandresponsesurface experiments，andthescaled-upprocesswasvalidated inafermenter. Meanwhile，changes in chemical composition and sensory quality of tobacco with a -amylase treatment were analyzed. After optimization，the optimal fermentation medium and conditions for strain fermentation were determined as： corn meal 65g/L， soybean cakemeal 70g/L ，NaC1 10g/L ， CaCl2 （204 3g/L ，fermentation time 52h ，temperature 40°C ， pH6.0 ，inoculation volume 3% .The optimized a -amylaseactivityreached 1880.1U/mL . Compared with 30.6U/mL before optimization，the enzyme activity increased by 61.4-fold changes， and the enzyme activity could achieve 2276.2U/mL in 5L fermenter. After 7U/g （2号 a -amylase treatment， the starch content of tobacco decreased by 19.9% ，the total sugar and reducing sugar content increased by 8.09% and 7.81% ，respectively. The aromaprecursorsubstancesandotherindicatorswerepositivelyimprovedinvaryingdegres.Thetreatedtobaccoexhibitedenanced aromarichness，mootherandmildersmoke，withreducediitancyndof-otes.Theoptimedfermentationprocessignificantly improvedtheenzyeyieldofgeneticallyengineredstrainA8-1，demonstratingstrongindustrialaplicationpotential.Its implementation in cigarete production effectively modifies chemical composition and elevates smoke quality.
Keywords： a -amylase; fermentation; tobacco; application
淀粉是烟草中一类重要的碳水化合物，其含量与卷烟的感官品质有密切关系。（剩余10973字）
