晾晒烟腺毛分泌物抗病毒活性成分鉴定与评价
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中图分类号：S572.03；S435.72 文献标识码：A文章编号：1007-5119（2025）05-0010-10
Identification and Evaluation of Antiviral Components in Glandular Trichome Secretions of Sun/Air-cured Tobacco
TAN Yating， QI Chaofan1，2， DU Yongmei1， ZHANG Hongbo1， YANG Ying1， HAN Xiaol，FU Qiujuan1， OUYANG Yiming1，²，LIU Keqiang³*，LIU Yanhual* （1.Tobacco Research Institute，Chinese AcademyofAgricultural Sciences，Qingdao，Chia; 2.Graduate Schoolof Chinese AcademyofAgculturalSiences，Beijing1Cina;3.HeilongjiangbaccoIdustryCo，td.，Harbinina）
Abstract： Inordertoeucidate thecontrolefectofglandulartrichomesecretionsfromsun/air-curedtobacoonplantviradiseases andtoidentifysuperioractivecompoents，thissudyassedtheanti-MVactivityfethanolextractsfromglandulartichomesf 28 sun/air-cured tobaccogermplasmresources using asaprubing-inoculation method.Inadition，thecomposition andcontentof diterpenoidsandsucroseesters （SEs）intheglandulartrichomesecretionsweredeterminedbyultra-high performanceliquid chromatography（UPLC）andgaschromatographymassspectrometry（GCMS）.Corelation，partialleastsquares，andcustering analyseswereusedtoinvestigatetherelationship between thecontentof glandular trichomesecretionsandtheanti-TMVactivity. Theresultsshowedthat： （1）Theethanolicextractsof glandular trichomesfrom19germplasmsamples exhibited antiviral activity against TMV， with Q16 （Meitan sun-cured tobacco） showing the best virucidal efect， inhibiting necrotic spots by 68.87% ; （2） There wasa significant or extremely significant negative correlation between cis-abienol and a -cembratriene-diol， β -cembratriene-diol，EI， andSEII.Coveselyisldsiantorelysiatpoieoelaoit，SVd importantlycbol，Ediedgligianposiieelaioiteoateoict. （3）Cis-abienol，SE，andEVrentifiedasteajoactiveomponentsagaistinteladulartrcometisof sun/air-cured tobacco.Additionally， the validation experiments showed that cis-abienol （200μg/mL） and SEs （300μg/mL ）exhibited themost antiviral activities，with necrosis inhibition rate of 44.32% and 53.19% respectively.These rateswere significantly higer than those achieved by a plant-derived pesticide （ 0.5% amino-oligosaccharides）.The research results provide a material foundation and technical supportforthe efficient utilization and the development of plant-derivedantiviral activecomponents.
Keywords： sun/air-cured tobacco; glandular trichome secretions; TMV; active components
病毒病是农业生产上仅次于真菌病害的第二大植物病害，有“植物癌症”之称。（剩余13836字）
