抗青枯病雪茄烟ZX145的SNP指纹图谱构建

打开文本图片集

中图分类号：S572.03 文献标识码：A文章编号：1007-5119（2025）05-0001-09

SNP Fingerprint Construction and Bacterial Wilt Resistance ldentification of Cigar Tobacco ZX145

LI Guangcan 1，2 ， ZHANG Yuqin³， CAO Changdai³， ZHANG Xingwei²， XIAO Zhiliang²， YANG Qing4， ZHOU Dongxin5， ZHANG Huifen²， ZHOU Yong³， GENG Ruimei²， XIU Qigui5， SONG Zhimei6， HU Haizhou6*， YANG Aiguo1，2*

（1.ColegeofAgronomyingdaoAgriclturalUiversityQingdao266109，Chia;2.IstituteofobacoResearchofeAcademyofAgricultural Sciences，Qingdao266101，China;3.Shandong RizhaoTobaccoCo.，Ltd.，Rizhao276826，Shandong，China; 4.Yunnan Qujing Tobacco CompanyLtd.，Qujing 655ooo，Yunnan，China;5.LongyanBranchofFujianProvincialTobac Company，Longyan 36400，Fujian，China; 6.Qingdao China Tobacco Seed Co.，Ltd， Qing dao 266100，China）

Abstract：Toidentifyhigh-qualitycigartobaccogermplasmresistanttobacterialwilt（Ralstoniasolanacearum）andprovide molecular markersforsubsequent breeding，this studyutilized GBS （genotyping-bysequencing）resequencingdatafrom19tobaco germplasm resources，including the cigar tobacoline ZX145.Thenumber of identified ZX145-specific SNP loci was 221.The specific SNPloci were screened forthe development of KASP（kompetitivealele-specific CR）molecularmarkers.Validationusing 71 tobacco germplasm resourcesof diversetypes yielded 28KASP marker pairscapable of distinguishing ZX145 from other germplasm.AspecificSNPfingerprintforZX145wasconstructedusing24lociselected tocoverallchromosomes.Fieldevaluations forbacterialwiltsistane，alogsidecomprehesiveasesentsofgronomicandyieldtraitsereonductedindiseasenser ofFujian，Guizhou，andAnhui Province.TheresultsshowedthatZX145exhibited moderatelyresistance-resistanceinthedisease nurseries，whichwassuperiortotheconventionalcontrolvarieties，anditsoutputvalueperformedbeter.ConsequentlyZX145 represents an eliteresistant germplasmresource forbreding bacterial wilt-resistant cigar tobaccocultivars.The developed KASP markers provide robust molecular tools to support its utilization in breeding programs.

（eywords： bacterial wilt; cigar tobacco; fingerprint map; disease resistance

青枯病是由青枯雷尔氏菌Ralstoniasolanac-earum引起的一种土传细菌病害，流行于热带及亚热带地区[1]。（剩余14857字）