中华BMP4基因的分子特征及表达研究

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中图分类号：S917.4 文献标识码：A 文章编号：1006-060X（2025）11-0086-07

Abstract：Toexplore themolecularcharacteristicsofBMP4inPelodiscus sinensisanditsroleinfoliculardevelopment，we employed molecularcloningand bioiformatics methodstoobtaintheful-lengthcDNAoftheBMP4 gene in this species.Furthemore， quantitativePCR wasemployedtoaalyzethetissue-specifcexpressonofthisgeneandtheexpressonpates intheovaryduring thereproductivecycleandtheembryonic gonads.Theful-engthcDNAsequenceofthis genewas1743bp，encompassinga45-bp 5'-UTR，a75-bp3-UTR，anda1209-bpopenreading frame （ORF）that encoded40aminoacidresidues.Thededuced protein had the isoelectric point （pI） of 9.24 and the molecular mass of 45.07kD .The amino acid sequence of BMP4 contained a signal peptide composedof24aminoacidresiduesand6helicalstructures.Thephylogeneticanalysisoftheaminoacidsequencesshowedthat te BMP4 of P. sinensisclusteredwiththoseofChelonia mydas，Carettcarett，andDermochelyscoriacea.ThemRNAlevelofBMP4 in the ovarian tissue washigher than those in the liver，muscle，intestine，and testis tissuesamples （P<0.05） .During thereproductivecycle of P. sinensis，thelowestmRNAlevelofBMP4wasobservedinwinter，whilethehighestmRNAleveloccurredduringthefollcular developmentstage inspring.Followingovulation，themRNAlevelofBMP4declined.Theresultssuggst hatBMP4isinvolvedin regulating the growth，maturation，and ovulation of ovarian follicles in P. sinensis.Inaddition，BMP4 may be involved in embryonic gonad development， but not in embryonic gonad differentiation.

Keywords：Pelodiscus sinensis;BMP4; gonad development; follicular development

排卵量是反映动物繁殖性状的关键性指标。（剩余9204字）