青稞HvnLEA6蛋白生物信息学和亚细胞定位研究

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Abstract：Thelateembryogenesisabundant protein （LEA）familyplaysasignifcantrole inseeddevelopmentandstressresistaceof plants.This studyemploedbioiformaticstoolsandthesubcellularlocalizationmethod toexplorethetructureandfunctionofLEA familymember，HvnLEA6，inHordeumvulgareL.var.nudumHook.f.TheresultsidicatedthatthepromoterregioofHnLEA6 contained multiple elements related toabscisicacid （ABA），jasmonicacid （JA），uxin，andsed-specificresponses.HA6 consistedofl17aminoacidresidues，washydrophilic，lackedasignalpeptideandtransmembranestructureandwaslocaledinthe nucleus.In the secondary structure ofHvnLEA6， a -helicesandrandom coilsaccounted for 62.39% and 37.61% ，respectively，with no extended strands or β -sheets.HvnLEA6possessedaconservedLEA_1domain，whichwasan a -helixandrepresentedthemost conservedstructureinHvnLEA6.Theproteinsurfaceregions withpositiveandnegativechargeswere alsoprimarilylocatedwithin theLEA_1domain.Therefore，thisdomain wasconsidered themain functionalregionofHvnLEA6.Moleculardynamicssimulations have revealed that HvnLEA6 exhibited high structural flexibility in the transition zone （aa75-85）between a -helices and random coils. Inconclusion，HvnLEA6maybedirectlyinvoledinprotectingthenucleicacid-proteinstructures withinthenucleusagaiststress conditions and the activity or expression is jointly regulated by ABA and JA.

Keywords：HordeumvulgareL.var.udumHook.f.;lateembryogenesisabundantprotein;HvnLEA6;bioinformaticsanalysis

青稞（HordeumvulgareL.var.nudumHook.f.）属于禾本科大麦属作物，是大麦的一种变种，又称裸大麦，具有悠久的栽培历史，是我国西北西南等高原地区重要的农作物之一[1-2]。（剩余10227字）