大豆短期连作对土壤理化性质和微生物多样性影响

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中图分类号：S153 文献标识码：A 文章编号：1006-060X（2025）11-0018-07

Abstract：Thisstudyaims toinvestigatetheimpactofshort-termcontinuoussoybeancroppngonsoilphysicochemicalproperties and microbial community structure.Rhizosphere soil samples were colected from the fields with new soybean cropping （CK）， continuoussoybeancroppingfor2years （T1），andcontinuoussoybancroppingfor3years（2）.High-throughputsequencing was combinedwithphysicohemicalpropertyanalysis toelucidatethekeydrivingfactorsbehindtheobstaclesasociatedwithshort termcontinuoussoybeancropping.Theresultsshowedthatsoilnutrientsexhbitedacharacteristicpateoffirstincreasinganden decreasing.The content of available phosphorus （AP） elevated in T1 and declined to 6.73mg/kg （only 42.06% of that in CK） in T2. The content of available potassium （AK） and organic matter （OM） in T2 decreased by 38.68% and 10.59% ，respectively， compared withthatinCK.esoilpaluedoppedto6.15.emicroialdiversityndichessicreasedin.oweverinT2otdic decreased to the levels comparable to those in CK. The relative abundance of Ascomycota reached 39.43% ，decreasing by 21.54 percentagepoitscompared withthatinCK，whilethelativeabundanceofMortierelomycota increasedby2.24percentagepoints. Therelativeabundanceofcidobacteriotarmainedstable，whiletherelativeabundanceofProteobacteriaincreasedby3.83prcentage points compared withthatinCK.Thecorelationanalysis showedthat terelativeabundanceofAscomycotahadsignificantlypositive correlations withAKandpH，while thatofMortierellomycota waspositivelyoelatedwithtotal posphorus（T）butnegatively correlatedwithOM，AK，andpH.Terelativeabundaneocidobacterihadsignifcatlypositiecoelations wittotalota （TK）and pH but significantly negative correlationswith HN and AP.TherelativeabundanceofProteobacteria had significantly positive correlationswith TP， TK，and HNbut significantly negative correlationswithTN，OM，andpH.Therelativeabundanceof Chloroflexi had significantly positive correlationswith OM and AK. Inconclusion，under short-term continuous soybean cropping， the

AP content below 10mg/kg and the relative abundance ofAscomycota below 40% are often accompanied by soil quality deterioration andmicrobialcommunityimbalance，ndthustheycanbetakenasthewarningindicatorsofshort-tecontinuouscroppingobstacles. Key words：soybean; continuous cropping obstacles; soil nutrients;soil microorganisms; microbial community

长期单一种植会引起连作障碍，导致产量下降、土传病害发生率升高以及土壤生态功能退化等问题[1-2]。（剩余11813字）