高原鼠兔干扰对高寒草甸土壤古菌群落特征的影响

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中图分类号：S812.2 文献标识码：A 文章编号：1007-0435（2025）12-3994-10

doi：10.11733/j.issn.1007-0435.2025.12.012

引用格式：，等.高原鼠兔干扰对高寒草甸土壤古菌群落特征的影响[J].草地学报，2025，33（12）：3994一 4003 WANG Si-yu，LILin，CHEN Ke-long，et al.Efectsof Plateau Pika Disturbanceon Soil Archaea Community Char acteristics inAlpineMeadow[J].Acta Agrestia Sinica，2025，33（12） ：3994-4003

EffectsofPlateau Pika Disturbance on Soil Archaea Community CharacteristicsinAlpineMeadow

WANG Si-yu， LI Lin， CHEN Ke-long， ZHANG N i， SUN Jian-qing5， SHI Qian-qian

（1.CollegeofLifeiencesinghaiNoalUniversityXiningQighiProvice88，China;2.QinghiProviceKeyLabatoyf PhysicalGeogandotalrsslgaicalceigialUesityiinire 810008hina;3.KeyLaboratoryofbetanateauandSurfaceProcesssandEologicalCoseatioistryofducatio）igi NormalUniversityXiingQinghaiProvceo，Cha;4NtialPositioingObsevatioadRseachSatfQigaiLakeWetad EcosystemiiilFddstgoei；l NatureReserveAdministration，Gangcha，QinghaiProvince8123oo，China）

Abstract：As a unique mammal in the alpine meadow ecosystem of the Qinghai-Tibet Plateau，the disturbance caused by plateau pikas （Ochotona curzoniae） may accelerate alpine meadow degradation The degradation of the alpine meadow has a significant afects on the soil archaeal communities.In this study，we investigated the characteristics of soil archaeal communities under pika disturbance by analyzing surface soil （0-10cm，ST1. and subsurface soil （ ：10-20cm ， ST2 ）through high-throughput sequencing of archaeal 16S rRNA. The results revealed that Thaumarchaeota remained the dominant archaeal phylum across alltreatments，with Candidatus

Nitrososphaera as the predominant genus. Compared to natural conditions，pika disturbance increased archaeal diversity in surface soil butdecreased it in subsurface soil.LEfSe analysis demonstrated that pika activity significantly enhanced the abundance of three nitrogen-cycling taxa in subsurface soil： Candidatus Nitrososphaera， Marine Group II，and Thermoplasmata.In conclusion，plateau pika activities alter the structure of soil archaeal communities in alpine meadows，and the impact on the surface soil was more obvious.

Key Words：High-throughput sequencing;Tibetan Plateau; Thaumarchaeota;Candidatus Nitrososphaera

青藏高原的高寒草甸面积约占中国草地总面积的 44%[1] ，是我国草地生态系统的重要组成部分2。（剩余20387字）