MgSO4 胁迫对紫花首蓿生理响应及基因表达的影响
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中图分类号：S541.9 文献标识码：A 文章编号：1007-0435（2025）12-3944-14
引用格式：． MgSO4 胁迫对紫花苜蓿生理响应及基因表达的影响[J].草地学报，2025，33（12）：3944-3957WEIXiao-na，ZHANGXu-ze，SHEN Ying-fang.The Effectsof MgSO4 Stress on the Physiological Responses andGeneExpression ofAlfalfa[J].Acta Agrestia Sinica，2025，33（12） ：3944-3957
TheEffectsof MgSO4 Stress on the Physiological Responsesand Gene ExpressionofAlfalfa
WEI Xiao-na， ZHANG Xu-ze， SHEN Ying-fang
（1.TheColgofologicalEviotdesourcesQgiinuUesityingQiircei；.KbtorofResoureCstrydEovioetalrotetioiQghitatea（QighiinuUesitytateEcismission，Xiigingiroceina；QihircialKeybotoryofgueUtltioofracterstc Plants，Xining，Qinghai Province 81ooo7，China）
Abstract：To investigate the effects of MgSO4 stress on the physiological responses and gene expression of alfalfa（Medicago sativa L.），the‘Zhongmu No. 1⋅ alfalfa variety was used as the test material in this study. A hydroponic method was employed to simulate salt stress treatments by seting up six concentrations of MgSO4 （0，40，80，120，160，and 200mmol⋅L-1⟩ .The germination indices of alfalfa seeds，the growth and physiological indices of seedlings，and the expresson levels of salt-tolerance-related genes were determined. The results showed that as the concentration of MgSO4 increased，the germination and growth of alfalfa seedlings were inhibited，the contents of chlorophyll，proline，soluble protein，soluble sugar，malondialdehyde，and theactivity of superoxide dismutase in seedlings were increased.The root-shoot ratio and the activities of per oxidase and catalase was first increased and then decreased.Byanalyzing of the expression levels of salt-toler ance-related genes in alfalfa，it was found that genes related to enzyme activity，photosynthesis，flavonol syn thesis，wax synthesis，and brassinolide synthesis pathways may play important roles in the tolerance of ‘ Zhongmu No. 1∘ alfalfa seedlings to MgSO4 stress.In conclusion，‘Zhongmu No. 1⋅ alfalfa has the potential to be cultivated on saline-alkali land with MgSO4 content less than or equal to 200mmol⋅L-1 and pH less than 9.0.
Key words： MgSO4 ;Germination of seeds ;Growth and physiological indicators ;Expression of genes
气候变化和不当的灌溉方式导致全球干旱和半干旱地区产生严重的次生盐碱化问题1。（剩余23001字）
