基于IlluminaMiSeq高通量测序分析玉米种子携带真菌多样性及功能

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中图分类号：S435.13 文献标识码：A 文章编号：1007-0435（2025）12-3907-13

doi：10.11733/j.issn.1007-0435.2025.12.005

引用格式：，等.基于Illumina MiSeq高通量测序分析玉米种子携带真菌多样性及功能[J].草地学报， 2025，33（12） ：3907-3919 DUANYu，JIN Meng-jun，FENG Zhong-hong，et al.Analysisof Fungal DiversityandFunction in Maize Seeds Based on Ilumina MiSeq High-Throughput Sequencing[J].Acta Agrestia Sinica，2O25，33（12） ：3907-3919

Analysis of Fungal Diversity and Function in Maize Seeds Based on Ilumina MiSeq High-Throughput Sequencing

DUAN Yu， JIN Meng-jun， FENG Zhong-hong，XU Yong-feng，YANG Cheng-del* （1.CollegeofantrtetioiclualUiesitcoolEingbotoisessndstsfso， Lanzhou，GansuProvince730O，China;2.GansuZhangyePlantProtectionandIspectioStationZhangye，GnsuProvice734ooChina）

Abstract：Seeds are fundamental resources in agricultural production，and the fungi they carried on the surface can be vertically transmited into host plants，affecting plant health and crop quality.In this study，seeds from six maize（Zea mays） varieties （lines）were analyzed using Illumina MiSeq high-throughput sequencing to investigate the diversityand potential functions ofseed-borne fungi.The fungal communities were mainly composed of members from Ascomycota，Basidiomycota，and Mucoromycota，with Ascomycota being dominant. Hypocreales and Mucorales were the predominant fungal orders，and the dominant genera included Fusarium， Rhizopus，and Penicillium. Significant differences in fungal community structureand diversity were observed among different maize varieties （ ⋅P<0.05 ），with Fusarium being more abundant in‘Mingtian 695’than in ‘Deguang l68’. Seeds production year also significantly influenced fungal composition. The seeds produced in 2023 had the lowest Fusarium abundance，while those from 2O21 had the highest infection rate.Alpha diversity，beta diversity，and LEfSe analyses further revealed distinct community structures among the varieties.

Functional predictions identified 68 KEGG metabolic pathways and 27 ecological functions，with pathotrophsaprotroph types being most dominant. Extended storage time increased the abundance of pathogenic fungi and ffected energy metabolism pathways. These findings provide a theoretical basis for understanding seed-borne fungal diversity in maize，assessing seed health，and developing targeted fungicides.

Key words：Maize seed zone fungi;Iumina MiSeq high-throughput sequencing;Fusarium;Functional prediction

种子作为植物的繁殖核心，是农业生产中不可或缺的“芯片”资源，在农业发展中占据着重要地位[1]。（剩余20563字）