泡果茜草叶绿体基因组序列特征分析

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中图分类号：Q943 文献标识码：A 文章编号：1007-0435（2025）12-3933-11

引用格式：，等.泡果茜草叶绿体基因组序列特征分析[J].草地学报，2025，33（12）：3933－3943 YU Wei，ZHOU Xin-xin，YANGLi-e，et al.Analysis of the Sequence Characteristics of the Chloroplast Genome of Microphysa elongata[J]. Acta Agrestia Sinica，2025，33（12） ：3933-3943

Analysis of the Sequence Characteristicsof the Chloroplast Genome of Microphysa elongata

YUWei，ZHOUXin-xin，YANGLi- ⋅e ，PENG De-lil

（1.CollgeofienalUiesityugaroea;othacaldeAcademyofScieesangouuagdogrovice5hina;KeyaboatoryofatioalFrestrydGasanddonPlantConservationandUtilizationinSoutheChinaGuangzou，GuangdongProvice5O5O，China;4.FacultyofGeogaphyuan NormalUniversity，Kunming，YunnanProvince65o5oo，China）

Abstract：This study utilized Illumina high-throughput technology sequenced the chloroplast genome of Micro physa elongata （Schrenk） Pobed.and annotated its chloroplast genome.The structural features，and its phylo genetic relationships were analyzed！The results show that the Microphysa genus possesses a typical quadripar tite structure，with a genome length of155 O87bp.Among them，the large single-copy region（LSC） is 85 399 bp，the smallsingle-copy region（SSC）is1711O bp，and both inverted repeat regions（IR）are 26 289 bp in length.This genome contains 87 protein-coding genes，37 transfer RNA（tRNA）genes，and 8 ribosomal RNA（rRNA） genes.Variations in the lengths of rps19，ndhF，and ycfl genes，along with their distances from the boundaries，lead to diferences in the IR regions among Pseudogalium and Microphysa. The degree of variation innon-coding regions is significantly greater than that in coding regions.A total of 6Odispersed repeat sequences，86 simple sequence repeat （SSR） loci，and 27 O29 codons were identified in Microphysa elongata， with the most common codon being UUU，which encodes phenylalanine（Phe），and a preference for A/Uending codons.Phylogenetic analysis indicates that Microphysa elongata forms an independent clade and is nested within the broadly defined genus Galium.The results of this study could provide references for further study on genetic structure and diversity，specific gene mining，and phylogenetic studies of the tribe of Rubieae. Key words：Microphysa elongata（Schrenk） Pobed;Chloroplast genome;Sequence structure;Phylogenetic analysis

泡果茜草[Microphysaelongata （Schrenk）Pobed.]隶属于茜草科茜草族胖果茜草属（MicrophysaSchrenk），又称俘果茜草，该属为单型属。（剩余16016字）