Pb、Cd胁迫下 CaCl2 与24-EBL对菊苣幼苗的缓解效应

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引用格式:,等.Pb、Cd胁迫下 CaCl2 与24-EBL对菊苣幼苗的缓解效应[J].草地学报,2025,33(11):3827—3836YUAN Xin-yue,LIU Yu-heng,YU Bao-gang,et al. The Alleviating Effects of CaCl2 and 24-EBL on Chicory Seed-lings underPb and Cd Stress[J].Acta Agrestia Sinica,2025,33(11) :3827-3836
中图分类号:Q94 文献标识码:A 文章编号:1007-0435(2025)11-3827-10
The Alleviating Effects of CaCl2 and 24-EBL on Chicory Seedlings under Pb and Cd Stress
YUAN Xin-yue LIU Yu-heng, YU Bao-gang, QIN Xiang-yu ,LITie LIU Li-jie, JIN Zhong- ⋅min ,HAO Yu
(1.CollgeofLifeScieneandgricultureandForestryQiiarUniversity,Qiqiar,HeilongjangProvinceo6,China;2.Heojang
ProvincialKeyLaboratoryofResistanceGeneEnginerigandBiodiversityConservationnColdRegions,Qiqia,HeilongjiangProvince 161006,China;3.Hainan Geological ComprehensiveSurvey andDesign Institute,Haikou,Hainan Province57O206,China)
Abstract:To investigate efficient and environmentally friendly strategies for mitigating lead (Pb)and cadmium (Cd)stress in plants,this study integrated calcium chloride( CaCl2 )and24-Epibrassinolide(24-EBL)to establish a combined intervention system based on“calcium signal regulation plus plant hormone regulation". Chicory(Cichorium intybus L.)subjected to Pb and Cd stress was expected to exhibit enhanced tolerance through the synergistic efects of this dual regulation.The experiment optimized three treatmentcombinations : Treatment B 'CaCl25mmol⋅L-1+24⋅EBL0.06mg⋅L-1) ,Treatment C (CaCl210mmol⋅L-1+24⋅EBL 0.04mg⋅L-1) ,and Treatment D (CaCl210mmol⋅L-1+24⋅EBL 0.06mg⋅L-1) . Under varying levels of Pb (204号 and Cd stress,chicory plants were subjected to Treatments B, C,and D,respectively,and their physiological parameters were subsequently analyzed.The results showed that the three treatments varied in their effectiveness in reducing Pb and Cd accumulation across different plant tissues and in enhancing stress resistance. Grey relational analysis demonstrated that Treatment B (CaCl25mmol⋅L-1+24⋅EBL0.06mg⋅L-1) was the most effective in alleviating Pb- and Cd-induced oxidative damage in chicory seedlings. It significantly reduced Pb and Cd concentrations in chicory,enhanced the non-enzymatic antioxidant system,and promoted polyphenol synthesis.
Keywords:Chicory;24-EBL; CaCl2 ;Heavy metal stress
重金属污染对药用植物的开发利用影响巨大,如何有效降低药用植物中的重金属积累问题已引发广泛关注,药用植物的持续供给及其安全性、有效性也成为近年来的研究热点[1]。(剩余18470字)