不同酶制剂降解玉米秸秆中木质纤维素效果的研究

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中图分类号：S816.5 文献标识码：A 文章编号：1007-0435（2025）11-3820-07

引用格式：.不同酶制剂降解玉米秸秆中木质纤维素效果的研究[J].草地学报，2025，33（11）：3820－3826QI Hong-hui， GAO Shu-xin，XUE Shu-yuan.Study on the Degradation Effects of Diferent Enzyme Preparations onLignocellulose in Corn Straw[J].Acta Agrestia Sinica，2025，33（11） ：382O-3826

Study on the Degradation Effects of Different Enzyme Preparations on Lignocellulose in Corn Straw

QI Hong-hui1，GAO Shu-xinl*，XUE Shu-yuan² （1.ColegeofAnimalScienceand Technology，InnerMongolia Minzu University，Tongliao，InnerMongoliaO280oo，China; 2.InnerMongoliaAgricultural and Animal Husbandry Academy，Hohhot，InnerMongolia Oloo31，China）

Abstract：The objective of this study was to investigate the impact of exogenous enzyme preparations on fiber degradation eficiency in corn straw.Corn straw was selected as the raw material，with four groups established ： a control group without enzymatic treatment，and thre experimental groups treated with 1g⋅kg-1 glucanase （Group 1）， 1g⋅kg-1 cellulase 1（Group 2），and 1g⋅kg-1 cellulase 2（Group 3），respectively. The results demonstrated that al experimental groups supplemented with exogenous enzymes exhibited a positive effect on lignocellulose degradation in corn straw.Compared to the control group，the acid detergent fiber（ADF） content and neutral detergent fiber（NDF） content in the three experimental groups decreased significantly （ P< 0.05），while the crude fat content and soluble carbohydrate content increased significantly （ P<0.05 .Based on the OPLS-DA model，a total of 18 metabolites with significant differences （ ⋅P<0.05 ）were identified between the control group and Group 3，which showed the best performance in lignocellulose degradation according to routine nutrient detection. The main metabolic pathways included biosynthesis of plant secondary metabolites，ABC transporters，and nucleotide metabolism.In conclusion，the addition of celulase 2 effectively reduced lignocellulose content，providing a theoretical foundation for the applicationof exogenous enzyme preparations in corn straw. Key words：Corn straw;Lignocellulose ; Enzyme preparation; Palatability;Metabolic pathway

近年来，随着粮食资源的日益紧缺，玉米秸秆已成为反刍动物粗饲料的重要来源。（剩余12372字）