饲用燕麦重要农艺性状的全基因组关联分析和 候选基因预测

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中图分类号：S322.6 文献标识码：A 文章编号：1007-0435（2025）11-3526-14

引用格式：，等.饲用燕麦重要农艺性状的全基因组关联分析和候选基因预测[J].草地学报，2025，33（11）： 3526-3539 LIU Xiao-xia，ANWen，LILin，et al.Genome-wide AssociationAnalysis and Candidate Gene Predictionof Impor tant Agronomic Traits of Forage Oat[J].Acta Agrestia Sinica，2025，33（11） ：3526-3539

Genome-wide Association Analysis and Candidate Gene Prediction of Important Agronomic Traits of Forage Oat

LIU Xiao-xia ， AN Wen LI Lin， ZHANG Xin-yu  ， LANG Si-rui MA Qiao-li，MA Dong-mei

（1.CultivatioseftateKybotorstLandgdtiodotetortiongiaUsitya Ningxia50o21，China;2.KeyLaboratoryofestorationandReconstructionofDegradedEcosystemsiNorthwestChina，Ministryof

Educatioodti

4.SchoolfigiUiitcaibtostruaoto UtilizationofeiaicalousstegiUesitycangiia；.olt and Grassland，Ningxia University，Yinchuan，Ningxia 75Oo21，China）

Abstract：Forage oat（Avena sativa L.）plays a crucial role in grassland animal husbandry and ecological sustainable development，yet the genetic mechanisms of its agronomic traits remain poorly understood. In this study，genome resequencing of 69 oat accessions was performed，yielding 47 993 754 high-quality SNPs for genome-wide association analysis （GWAS）of nine key agronomic traits.A total of 21O significantly associated SNPs were identified，distributing across all chromosomes，with the highest density on chromosome 3C.Further analysis revealed 120 candidate genes，including receptor kinase genes （ORK45，LRK45） and the E3 ubiquitin ligase gene At1g63170 associated with plant height，both known to regulate height in other species. Traits such as flag leaf length，spikelet number per plant，and seed number per spikelet were linked to a zinc finger CCCH domain-containing protein gene，which played a role in plant growth and stress responses. Chlorophyll content was associated with ABC transporter genes， the HY5 transcription factor，and an ethylene-responsive transcription factor gene，and allinvolved in light response.Haplotype analysis of1O SNPs corresponding to16 key candidate genes showed 2-4 haplotypes per locus，with most exhibiting significant phenotypic variation. This study provides valuable genetic resources and a theoretical basis for future molecular breeding in forage oat. Key words：Forage oat; Agronomic traits;Genome-wide association analysis ;Candidate gene

燕麦（AvenasatiuaL.）为禾本科（Poaceae）燕麦属一年生粮饲兼用作物[1]，具有抗干旱、环境适应能力强、产量稳定等特点，在世界范围内广泛种植。（剩余18219字）