放牧强度对荒漠草原不同微生境土壤细菌群落组成及多样性的影响

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中图分类号：S812.2 文献标识码：A 文章编号：1007-0435（2025）11-3571-10

引用格式：崔乐，李治国，吕世杰，等．放牧强度对荒漠草原不同微生境土壤细菌群落组成及多样性的影响[J].草地学报， 2025，33（11） ：3571—3580 CUILe，LI Zhi-guo，LYUShi-jie，etal.Efectsof Grazing Intensityon SoilBacterial Communities Compositionand Diversity in DiffrentMicrohabitatsofDesertSteppe[J].Acta Agrestia Sinica，2O25，33（11） ：3571-3580

Effects of Grazing Intensity on Soil Bacterial Communities Composition and Diversity in Different Microhabitats of Desert Steppe

CUI Le，LI Zhi-guo*，LYU Shi-jie，DUAN Lei-yu，WANG Xin-yu， SUN Xue-yan （College ofGrassand Science，InnerMongoliaAgricultural University，Hohhot，InnerMongolia Oloooo，China）

Abstract：Inthis study，the efects of diferent grazing intensities on the composition and diversityof soil bacte rial communities in desert steppe were investigated.Three grazing intensities，light grazing （LG），moderate grazing（MG）and heavy grazing（HG），were set up，and no grazing area（CK） was as control. High-throughput sequencing of16S rRNA sequences of soil bacteria at the base of biological crusts，bare land and dominant species （Stipa breviflora and Cleistogenes songorica） was carried out to analyze the efects of grazing intensity on soil bacterial composition and Alpha diversity in different microhabitats.The results showed that：（1） Actinobacteria，Proteobacteria，Chloroflexi and Acidobacteria were thedominant bacteria at the phylum level，and Rhodobacter，Microcladium，RB41， Sphingomonas and Segetibacter were the dominant bacteria at the genus level.（2）Diferent grazing intensities had no significant effect on the ACE index，Chaol index and Shannon indexof soil bacteria in different microhabitats.The Simpson indexes of Stipa breuiflora basal soil and Cleistogenes songorica basal soil were significantly higher than those of biological crusts （ ⋅P<0.05） .（3）The results of RDA analysis showed that total carbon，total nitrogen，total phosphorus，TC/TN ratio， pH value and soil moisture were the driving factors of the changes of soil bacterial α diversity. These results would provide scientific guidance for achieving sustainable management of desert steppes. Key Words：Grazing;Desert grassland;Microhabitat;Soil bacterial community;Structure

荒漠草原作为一特殊生态系统，在维持生物多样性、促进生态平衡及提供生态系统服务方面意义重大]。（剩余15844字）