基于基因组Survey的翅果菊基因组大小评估及特征分析

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中图分类号：Q944 文献标识码：A 文章编号：1007-0435（2025）11-3540-10

引用格式：，等．基于基因组Survey的翅果菊基因组大小评估及特征分析[J].草地学报，2025，33（（11）：3540—3549ZHAO Chun-liu，ZHANG Bin，ZONG Mei，et al. Genome Size Estimation and Characterization of Lactuca indicaBased on Genome Survey Analysis[J].Acta Agrestia Sinica，2025，33（11） ：354O-3549

Genome Size Estimation and Characterization of Lactuca indica Based on Genome Survey Analysis

ZHAO Chun-liu， ZHANG Bin， ZONG Mei， ZHAO Hong， XUE Ying-fei，WANG Chen-chen， LIU Yang-yang， GAO Pu ， SUN Cheng，LI Da-yong

（1.CollgeofeieiallUisite，i；2tolEeeatefoble，BeijingVgetableRseachCeterejingcadyofgrictureadFrestrieceeo7a;3BKytofCropMolecuaigndtellgeteinei;leifcdolUi Shandong Province 250014，China）

Abstract：Lactuca indica is a plant species and valued for its medicinal，edible and forage properties.Itdemonstrates high biomassproductionand significant environmental adaptability，with considerablepotential asa highquality forage crop.However，the limited availability of genomic information on L .indica hashindered the effctive use of its genetic resources and molecular breeding. In this study，we estimated the genome size and evaluated the genomic characteristics of L . indica using flow cytometry and genomic survey methods. Chromosome counting analysis confirmed the somatic chromosome number of 2n=18 . Flow cytometry estimated the genome size of L . indica was between 4.26 and 4.5O Gb. Next-generation DNA sequencing technology and

K-mer-based analysis further suggested that its genome size was approximately 4.76Gb ，with a heterozygosity of 0.24% . Repetitive sequences accounted for 73.76% of its genome.The preliminary assembly of the L ： indica genome yielded a total length of 4.02Gb with a contig N50 of 5070 bp. This study provided a preliminary genomic characterization of L . indica， laying the foundation for further molecular breeding and enhancement of forage traits.

Key words：Lactuca indica;Genome size;Genome survey;Forage crop;K-mer analysis

翅果菊（Lactucaindica，异名为Pterocypselaindica），又称苦莴苣、山莴苣、山马草等，是一年或二年生的菊科（Asteraceae）莴苣属（Lactuca）草本植物。（剩余19712字）