野牛草BdGH3基因家族鉴定及干旱胁迫下的表达模式分析

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中图分类号：Q786 文献标识码：A 文章编号：1007-0435（2025）11-3515-11

引用格式：，等.野牛草BdGH3基因家族鉴定及干旱胁迫下的表达模式分析[J].草地学报，2025，33 （11） ：3515-3525 WANG Xue-ping，WANG Si-ning，QI Hao-le，et al.Identification of Buchloe Dactyloides BdGH3 Gene Family and Analysis of Expression Pattern under Drought StressJ]. Acta Agrestia Sinica，2025，33（11）：3515-3525

Identification of Buchloe Dactyloides BdGH3 Gene Family and Analysis of Expression Pattern under Drought Stress

WANG Xue-ping，WANG Si-ning，QI Hao- ⋅le， ZHANG Ran，LI Xiao-xia  ，MAHui-ling

（1.ColegofaculturalieeugricturalUvesityKeyboatoratacualoestrucatiatacu

turalEngnerigLbratorofsuPriceiUnterforaingandEosustaiabitLaosProce

China;2.Istitealsedstoadetsahta

GrasslandAistratioeinnssdegolicuralUvesitKeto Grassland Resources of the Ministry of Education，Hohhot，Inner Mongolia Olooll，China）

Abstract：Auxin-amido synthetases （Gretchen Hagen 3，GH3），as early auxin-responsive genes，play a key rolein regulating plant growth，development，and responses to abiotic stress.To identify GH3 genes in Buchloe dactyloides that respond to drought stress，this study used bioinformatics methods to identify 16GH3 family genes in the genome of Buchloe dactyloides‘honglinyu No.1'. The BdGH3 genes in B . dactyloides are unevenly distributed across 11 chromosomes. Motif and gene structure analyses revealed that most BdGH3 genes contain conserved motifs（Motifl to Motif1O）andO to 2 exons.Subcelllar localization predictions ndicated that fifteen BdGH3 proteins are localized in the chloroplasts.Phylogenetic tree analysis indicated that

BdGH3 genes are clustered into three subfamilies.Cis -acting element analysis showed that BdGH3 genes contain multiple cis-acting elements related to light signals，hormone signals，and drought stress.Expression pattern analysis revealed that the expresson levels of BdGH3.1 and BdGH3.15 in leaves，and BdGH3.5 and BdGH3.9 in roots，were significantly up-regulated throughout the drought stress period.The results of this study provide a theoretical reference for further functional research on BdGH3 genes and the drought - response mechanism in B. dactyloides.

Key words：Buchloe dactyloides ; GH3 gene family identification; Drought stress;Expression pattern analysis

植物在生长发育过程中容易受到干旱、盐碱等非生物胁迫。（剩余18431字）