羊草LcPDS基因的克隆、表达模式分析及亚细胞定位

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中图分类号：S543.9 文献标识码：A 文章编号：1007-0435（2025）11-3550-09

引用格式：王介繁，李健辉，张生原，等.羊草 LcPDS 基因的克隆、表达模式分析及亚细胞定位[J].草地学报，2025，33（11）：3550-3558WANG Jie-fan，LIJian-hui，ZHANGSheng-yuan，etal.Cloning，Expression Patterm Analysis and SubcellularLocalization of LcPDS gene in Leymus chinensis[J].Acta Agrestia Sinica，2025，33（11） ：3550-3558

Cloning，Expression Pattern Analysis and Subcellular Localization of LcPDS gene in Leymus chinensis

WANG Jie-fan ， LI Jian-hui， ZHANG Sheng-yuan， DONG Di， ZHANG Ran， WANG Si-ning， LIU Yue-han，LI Xiao-xia，SHIFeng-ling

（1.KeyLaboratoyndsoucofistryucatioolorassndiec，ogolagricluraUsity Hohot，I8ia;QGutdiio;siteolc tionandRestoratio，ineseAcadyofFrestryeijingoChia;4rassndResearchndTecholoyPromotioart， Xinjiang Uygur Autonomous Region Grassland Station ，Urumqi，Xinjiang 83oooo， China）

Abstract： Phytoene desaturase （PDS），which affects the biosynthesis of carotenoids，is a key enzyme in the synthetic pathway. In order to study the function of the PDS gene of Leymus chinensis，in this experiment，the PDS gene was successully cloned from‘Zhongke No.1’L. chinensis and bioinformatics analysis was performed.Protein structure analysis，subcellular localization，transcriptional self-activation detectionand expression pattern analysis were conducted as well. The results showed that the ful-ength cDNA sequence of this gene is 1743bp ，encoding 58O amino acids. Chloroplast transit peptides may exist in the LcPDS protein，and thethe protein has the closest relationship with thePDS protein of wheat（Triticum aestirum）．Subcellular localization revealed that the protein is localized in thechloroplast.The transcriptional self-activation test showed that LcPDS does not have self-activation. There was no significant difference in the LcPDS gene expression among different developmental stages of leaves.The expression levels in various organs followed the order：root > leaf > stem（ P<0.05） .The LcPDS gene is regulated by gibberellin，abscisic acid，methyl jasmonate and salicylic acid. This study provides a scientific foundation for further research on the function of the LcPDS gene.

Keywords：Leymuschinensis; PDS gene;Bioinformatics;Analysis of expression patterns

植物类胡萝卜素作为重要的天然色素和光保护物质，在光合作用、光氧化防御及植物生长发育调控中发挥关键作用[1-2]。（剩余16397字）