基于 Nrf2/HO-1 信号通路探讨褪黑素对去卵巢大鼠氧化应激损伤的作用

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关键词：绝经后骨质疏松症；褪黑素；氧化应激；Nrf2/HO-1信号通路 中图分类号：R977.1文献标志码：A文章编号：2097-7174（2026）02-0112-06 DOI：10.3969/j.issn. 2097-7174. 2026.02.002

The effect of melatonin on oxidative stress damage in ovariectomized rats based on the Nrf2/HO-1 signaling pathway

LIN Yu，CHEN Qiyong，XIAO Lili，LIN Liqiong （Fuzhou Second General Hospital，Fuzhou，Fujian 350008）

Abstract：Objective：Toexplore the mechanismofmelatoninonoxidativestressdamage inratswithrapidbonelossafter ovariectomybasedontheNrf2/HO-1signaling pathway.Methods：Atotalof24SDratswererandomlydividedintoasham operation group，a model control group，anda melatonin-treated group，with 8rats ineach group.Bilateraloophorectomy was performed to replicatearapidbone lossmodel，followed by gastric intervention for12 weeks.Bone density was measuredusing adual energy X-rayabsorptiometry，and bonemicrostructurewas observed using hematoxylin-eosin staining.ELISA was used to detect bone metabolism indicators including bone-specific alkaline phosphatase（BAP）， tartrate-resistantacid phosphatase （TRAP），and oxidative stressindicators such as superoxide dismutase （SOD）and malondialdehyde（MDA）.Real-timePCR and Western blot were used to detect therelative mRNAand protein expression levels of nuclearfactorerythroid2-related factor2（Nrf2）and heme oxygenase-1（HO-1）in thefemur.Results ： Compared with thesham operation group，thebone mineraldensityofrats inthe modelcontrol groupdecreased significantly， indicating that therat modelof boneloss wassuccessfully established.After12 weeksof melatonin intervention， compared with the model control group，the BMD of the melatonin group was significantly increased （ P<0.01 ）；the numberof trabeculae increasedandthe trabecular spacing shortened；the serum BAPand TRAPwere both decreased （ P<0.01 ）；SOD was increased and MAD content was decreased （P<0.01 ）；and the mRNA and protein expression levelsof Nrf2and HO-1were significantly increased.Conclusion：Melatonincanactivate theNrf2/HO-1 signaling pathway，reduce oxidative stressdamage，and improvebone losscaused bya sharpdecrease in estrogen levels. Key Words：Postmenopausal osteoporosis；Melatonin; Oxidative stress；Nrf2/HO-1 signaling pathway

绝经后骨质疏松症（Postmenopausalosteoporosis，PMOP）是一种常见于绝经后女性的疾病。（剩余9962字）