胶质母细胞瘤类器官构建方式的改良及其在电场治疗研究中的应用

打开文本图片集

【中图分类号】 R739.41 【文献标志码】 A 【文章编号】1672-7770（2026）01-0030-06

Improved method for obtaining glioblastoma organoid and its application in research tumor-treating field WANG Siyuan， SHI Zhonggang， DAI Dawei， YE Zhenhong， MIAO Yifeng， QIU Yongming. Hospital， Shanghai

Corresponding author： QIU Yongming

Abstract： ObjectiveTo improve the method establishing glioblastoma organoids（GBO） and explore the feasibility evaluating the effect new treatments.MethodsThe postoperative pathological tissues glioblastoma（GBM） diagnosed as WHO grade IV were collected，and the tumor organoids and primary cells from different patientswere established and compared with the parental tumors，and the GBO were used to evaluate the efect tumor-treating field treatment in vitro.ResultsFifteen the eighteen GBM tissues were successfully established and retained the structural characteristics the parental tumors，which could be cultured for a long time and used for experiments. Primary GBM cells were obtained during the process，and expressed CD133 in immunluorescence staining.In vitro experiments confirmed that TTF treatment could inhibit the growth GBO. ConclusionsThis study improved a low-cost method to rapidly establish GBO to evaluate the effect tumor-treating field treatment.

Keywords：glioblastoma；organoid；primary cell； tumor-treating field

胶质母细胞瘤（glioblastoma，GBM）是成人最常见的中枢神经系统原发恶性肿瘤[1]，约占中枢神经系统原发恶性肿瘤的 50%[2] 。（剩余9084字）