基于“抗氧化-炎症-色素沉着轴"优化白芍的提取工艺与美白功效评价

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Abstract：ObjectiveTooptimizetheextractionprocessoftheactivecomponentsofRadixPaeoniaeAlbaandassessthe antioxidantanti-inflammatoryandpigmentatio-reducingefectsofRadixPaeoniaeAbaextract（BT）.Methodsoleular dockingidentifiedgalicacid，liquiritinandpeoniflorinaspotentialtyrosinaseibitorsandtheirontentsinwere determinedwithhigh-performanceliquidchromatography（HPLC）.Basedonthecontentsofthe3inibitors，theirtyrosinaseinhibitingactivityandDPPHradicalscavengingcapacitycalculatedusingentropyweightmethod，theextractioncoditions Wereoptimizedforsolventratio，liquid-to-solidratio，extractiontime，andparticlesize.TheinhibitoryeffectsofTon tyrosinase activity and melaninwere assessed in α -MSH-treated B16F10 cells，and itseffect onreactive oxygen species （ROS） production was evaluated in H2O2 -treated HaCaTcells. Ina mouse model of melanogenesisinducedby UVB radiation， the effect of BST gavage （100， 150，and 200mg/kg ）were evaluated by assessing skin conditions of the mice， Fontana-Masson staining，anddetectingserumlevelsofcytokinesrelatedtomelanogenesis，melanosometransport，metabolism，and inflammation. Results The optimized parameters for BST extraction included 50% ethanol， liquid-to-solid ratio of 15： 1， extraction time of 1h， and20-mesh particle size.BSTexhibited strong freeradical-scavengingactivity and significantly reducedmelanogenesisandincreasedtyrosinaseproteininB16cels，andloweredROSproductioninHaCaTcels.Inthe mousemodelsofmelanogenesis，BSTsignificantlysuppressdthemelanogenicenzymesandinflammatoryresponsesby reducing the levels of tyrosinase and TNF- α， thereby efectively reversed UVB-induced photoaging and hyperpigmentation. ConclusionTheoptimizedBSTextractionprocessisstableandpredictable.STshowsgoodskin-whiteningefect，whichis mediated posiblyby inhibitingtyrosinase activity andmodulating oxidativestressand inflammatorypathways.

Keywords：whitepeonyxtract;uvaateresponsesufacemethod;6cels;Hacatces;antioidanteect;siniteing

中药及中药复方在护肤领域的应用历史悠久，为中药养颜提供了药物选择、用药配伍等方面的基础支持[1]。（剩余17678字）